In situ measurement of fine root water absorption in three temperate tree species - Temporal variability and control by soil and atmospheric factors

Miniature heat balance-sap flow gauges were used to measure water flows in small-diameter roots (3-4 mm) in the undisturbed soil of a mature beech-oak-spruce mixed stand. By relating sap flow to the surface area of all branch fine roots distal to the gauge, we were able to calculate real time water uptake rates per root surface area (J(S)) for individual fine root systems of 0.5-1.0m in length. Study aims were (i) to quantify root water uptake of mature trees under field conditions with respect to average rates, and diurnal and seasonal changes of J(S), and (ii) to investigate the relationship between uptake and soil moisture theta, atmospheric saturation deficit D, and radiation 1. On most days, water uptake followed the diurnal course of D with a midday peak and low night flow. Neighbouring roots of the same species differed up to 10-fold in their daily totals of J(S) ( < 100-2000 g m(-2) d(-1)) indicating a large spatial heterogeneity in uptake. Beech, oak and spruce roots revealed different seasonal patterns of water uptake although they were extracting water from the same soil volume. Multiple regression analyses on the influence of D, I and theta on root water uptake showed that D was the single most influential environmental factor in beech and oak (variable selection in 77% and 79% of the investigated roots), whereas D was less important in spruce roots (50% variable selection). A comparison of root water uptake with synchronous leaf transpiration (porometer data) indicated that average water fluxes per surface area in the beech and oak trees were about 2.5 and 5.5 times smaller on the uptake side (roots) than on the loss side (leaves) given that all branch roots < 2 mm were equally participating in uptake. Beech fine roots showed maximal uptake rates on mid-summer days in the range of 48-205 g m-2 h(-1) (i.e. 0.7-3.2 mmol m(-2) s(-1)), oak of 12-160 g m(-2) h(-1) (0.2-2.5 mmol m(-2) s(-1)). transpiration rates ranged from 3 to 5 and from 5 to 6 mmol m(-2) s(-1) for sun canopy leaves of beech and oak, respectively. We conclude that instantaneous rates of root water uptake in beech, oak and spruce trees are above all controlled by atmospheric factors. The effects of different root conductivities, soil moisture, and soil hydraulic properties become increasingly important if time spans longer than a week are considered. (c) 2005 Gesellschaft fur Okologie. Published by Elsevier GmbH. All rights reserved.