Environmental metabonomics: Applying combination biomarker analysis in earthworms at a metal contaminated site

被引:103
作者
Bundy, JG
Spurgeon, DJ
Svendsen, C
Hankard, PK
Weeks, JM
Osborn, D
Lindon, JC
Nicholson, JK
机构
[1] Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London SW7 2AZ, England
[2] Ctr Ecol & Hydrol, Huntingdon PE28 2LS, England
关键词
histidine; maltose; pattern recognition; heavy metals; NMR spectroscopy;
D O I
10.1007/s10646-003-4477-1
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Earthworms were taken across an environmental gradient of metal contamination for ecotoxicology assessment. Both indigenous (Lumbricus rubellus and L. terrestris) and introduced earthworms (Eisenia andrei, exposed in mesh bags) were studied. Changes in the levels of small molecule metabolites in earthworm tissue extracts were analysed by H-1 NMR spectroscopy as a means of identifying combination biomarker compounds. Principal components analysis of the NMR spectral data revealed that biochemical changes were induced across the metal contamination gradient. Native worms (L. rubellus) from the most polluted sites were associated with an increase in the relative concentration of maltose; a decrease was also seen in the concentration of an as yet unidentified biomarker compound. Introduced worms (E. andrei) did not show differences to the same extent. Direct integration of the resonances from histidine and 1-methylhistidine showed that relative histidine concentrations were elevated slightly for L. rubellus, confirming the results of earlier mesocosm studies. Conversely, the relative concentrations of both histidine and I-methylhistidine were greatly reduced by metal contamination in L. terrestris. This study demonstrates the utility of NNIR spectroscopy in detecting previously unknown potential biomarkers for ecotoxicity testing and identified maltose as a potential biomarker compound deserving of further study.
引用
收藏
页码:797 / 806
页数:10
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