Bicarbonate permeability and immunological evidence for an anion exchanger-like protein in the red blood cells of the sea lamprey, Petromyzon marinus

被引:15
作者
Cameron, BA
Perry, SF
Wu, C
Ko, K
Tufts, BL
机构
[1] QUEENS UNIV,DEPT BIOL,KINGSTON,ON K7L 3N6,CANADA
[2] UNIV OTTAWA,DEPT BIOL,OTTAWA,ON K1N 6N5,CANADA
[3] NHLBI,CELL BIOL LAB,NIH,BETHESDA,MD 20892
关键词
anion movements; chloride/bicarbonate exchange; red cell proteins; AE1 or band 3; Petromyzon marinus;
D O I
10.1007/BF00263983
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Physiological and immune-blotting experiments were used to determine whether the red blood cell membrane of a primitive vertebrate, the sea lamprey Petromyzon marinus, contained a counterpart similar to the vertebrate anion exchange protein known as AE1 or band 3. Results of the physiological experiments which measured CO2 production after adding (HCO3-)-C-14 to the extracellular saline, indicated significant transmembrane bicarbonate movement in lamprey blood which unlike that in most vertebrates, was insensitive to inhibition by 4,4' diisothiocyanatostilbene-2,2' disulfonic acid. The present study also showed that lamprey red blood cells possess acetazolamide-sensitive carbonic anhydrase which is an important component of CO2 production by vertebrate red blood cells. Polyclonal immunoglobulins against a 12 amino acid domain in the C-terminus of the mouse AE1 recognized a trout red blood cell membrane protein with a relative molecular mass of 97 kDa, but failed to immunoreact with any membrane proteins from the red blood cells of lamprey. Antibodies against trout AE1 immunoreacted with trout red blood cell membrane proteins of approximately 97 kDa, 200 kDa and > 200 kDa. Interestingly, only a 200-kDa membrane protein from the red blood cells of the primitive lamprey immunoreacted with the trout anti-AE1 immunoglobulin proteins. Therefore, lamprey red blood cells appear to possess an AE1-like protein that may be physiologically different than that in most other vertebrates.
引用
收藏
页码:197 / 204
页数:8
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