Practical aspects of the measurement of the diffusion of proteins in aqueous solution

被引：39

作者：

Tillett, ML

Lian, LY

Norwood, TJ

机构：

[1] Univ Leicester, Dept Biochem, Biol NMR Ctr, Leicester LE1 7RH, Leics, England

[2] Univ Leicester, Dept Chem, Leicester LE1 7RH, Leics, England

来源：

JOURNAL OF MAGNETIC RESONANCE | 1998年 / 133卷 / 02期

关键词：

diffusion; PGSE; proteins; lysozyme;

D O I：

10.1006/jmre.1998.1472

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR diffusion measurements have been shown to be a useful tool for investigating the conformation and oligomeric state of proteins. Four main problems associated with making diffusion measurements of proteins in aqueous solution are identified and solutions proposed. The resulting experiment is demonstrated for an aqueous solution of hen egg white lysozyme. (C) 1998 Academic Press.

引用

收藏

页码：379 / 384

页数：6

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