Sequencing and functional analysis of the genome of Bacillus subtilis strain 168

被引：68

作者：

Harwood, CR

Wipat, A

机构：

来源：

FEBS LETTERS | 1996年 / 389卷 / 01期

关键词：

genome sequencing; gene function; gram-positive bacteria; yeast artificial chromosome; chromosome walking; integrational vector;

D O I：

10.1016/0014-5793(96)00524-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The international programme to sequence the 4.2 Mb genome of Bacillus subtilis, a model Gram-positive bacterium, is a joint project involving European, Japanese and US research groups. To date ca, 3.0 Mb of the genome has been sequenced, with the remaining 1.2 Mb expected to be completed in 1997. The amenability of B. subtilis to genetic manipulation, combined with the availability of extensive expertise on its biochemistry and physiology, makes this bacterium a valuable organism in which to investigate the properties of genes for which functions cannot be readily ascribed by standard methods.

引用

页码：84 / 87

页数：4

共 39 条

[1] Determination of a 17484 bp nucleotide sequence around the 39 degrees region of the Bacillus subtilis chromosome and similarity analysis of the products of putative ORFs [J].