Recombination of baculovirus DNA following lipofection of insect larvae

被引:6
作者
Hajós, JP
Zuidema, D
Kulcsár, P
Heldens, JGM
Závodszky, P
Vlak, JM
机构
[1] Agr Univ Wageningen, Dept Virol, NL-6709 PD Wageningen, Netherlands
[2] Hungarian Acad Sci, Inst Enzymol, Biol Res Ctr, Budapest, Hungary
关键词
D O I
10.1007/s007050050441
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
By injection of a liposomal complex containing baculovirus DNA directly into the hemocoel of insect larvae a polyhedrosis disease was induced. After cotransfection of insect larvae with circular viral DNA and transfer vector DNA recombinant viruses were generated with a frequency of about 2%, similar to what is obtained in vitro using insect cell cultures. Based on these results an alternative strategy for the generation of recombinants can be derived for baculoviruses for which susceptible cell lines are not readily available. This strategy involves the injection of baculovirus DNA into susceptible larvae followed by in vivo cloning.
引用
收藏
页码:2045 / 2050
页数:6
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