Burden of emerging anaerobes in the MALDI-TOF and 16S.rRNA gene sequencing era

被引:99
作者
La Scola, Bernard [1 ,2 ]
Fournier, Pierre-Edouard [1 ,2 ]
Raoult, Didier [1 ,2 ]
机构
[1] Univ Mediterranee, URMITE UMR CNRS IRD 6236, IFR48, Fac Med, F-13385 Marseille 5, France
[2] Assistance Publ Hop Marseille, Marseille, France
关键词
Anaerobes; 16S rRNA; MALDI-TOF; Sinusitis; Emerging anaerobes; RIBOSOMAL-RNA GENE; ASSISTED LASER-DESORPTION; FLIGHT MASS-SPECTROMETRY; IONIZATION-TIME; ROUTINE IDENTIFICATION; BACTERIAL IDENTIFICATION; CLINICAL-SIGNIFICANCE; BACTEREMIA; MICROBIOLOGY; CLOSTRIDIUM;
D O I
10.1016/j.anaerobe.2011.05.010
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The isolation of anaerobes from patients has declined in recent years, whereas their detection by molecular techniques has increased. In the present work, we analyzed the application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing to routine identification of anaerobes in clinical microbiology laboratory. We identified 544 isolates of 79 species by routine culture from deep samples in our hospital. MALDI-TOF MS allowed identification of 332 isolates (61%). The remaining 212 (39%) were identified by 16S rRNA gene sequencing, allowing identification of 202 at the species level. The most common anaerobes were Propionibacterium spp. (12%), Finegoldia magna (4%), Fusobacterium spp. (6%) and Bacteroides spp. (6%). However, among the 79 identified species, seven were new species or genera, including two Prevotella conceptionensis, a species previously detected by our team by amplification and sequencing, five Anaerococcus sp. and one Prevotella sp. Beyond the identification of these new species, we also identified several uncommon or previously not described associations between species and specific pathologic conditions. MALDI-TOF MS-based identification, which will become more effective with future spectra database improvement, will be likely responsible of a burden of emerging anaerobes in clinical microbiology. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:106 / 112
页数:7
相关论文
共 49 条
  • [1] Human Case of Atopobium rimae Bacteremia
    Angelakis, Emmanouil
    Roux, Veronique
    Raoult, Didier
    Drancourt, Michel
    [J]. EMERGING INFECTIOUS DISEASES, 2009, 15 (02) : 354 - 355
  • [2] Actinobaculum schaalii, a Common Uropathogen in Elderly Patients, Denmark
    Bank, Steffen
    Jensen, Anders
    Hansen, Thomas M.
    Soby, Karen M.
    Prag, Jorgen
    [J]. EMERGING INFECTIOUS DISEASES, 2010, 16 (01) : 76 - 80
  • [3] The possible significance of Clostridium spp. in blood cultures
    Benjamin, B.
    Kan, M.
    Schwartz, D.
    Siegman-Igra, Y.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (10) : 1006 - 1012
  • [4] BIZZINI A, 2010, J CLIN MICROBIO 0310
  • [5] ANAEROBIC OSTEOMYELITIS AND ARTHRITIS IN A MILITARY HOSPITAL - A 10-YEAR EXPERIENCE
    BROOK, I
    FRAZIER, EH
    [J]. AMERICAN JOURNAL OF MEDICINE, 1993, 94 (01) : 21 - 28
  • [6] RECOVERY OF ANAEROBIC-BACTERIA FROM CLINICAL SPECIMENS IN 12 YEARS AT 2 MILITARY HOSPITALS
    BROOK, I
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (06) : 1181 - 1188
  • [7] The role of anaerobic bacteria in sinusitis
    Brook, I
    [J]. ANAEROBE, 2006, 12 (01) : 5 - 12
  • [8] ANAEROBIC BACTERIAL BACTEREMIA - 12-YEAR EXPERIENCE IN 2 MILITARY HOSPITALS
    BROOK, I
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1989, 160 (06) : 1071 - 1075
  • [9] RECOVERY OF ANAEROBIC-BACTERIA FROM PEDIATRIC-PATIENTS - ONE-YEAR EXPERIENCE
    BROOK, I
    MARTIN, WJ
    CHERRY, JD
    SUMAYA, CV
    [J]. AMERICAN JOURNAL OF DISEASES OF CHILDREN, 1979, 133 (10): : 1020 - 1024
  • [10] BROOK I, 2009, ANAEROBE