Suppression of endogenous gene silencing by bidirectional cytoplasmic RNA decay in Arabidopsis

被引：138

作者：

Zhang, Xinyan ^{[1
]}

Zhu, Ying ^{[1
]}

Liu, Xiaodan ^{[1
]}

Hong, Xinyu ^{[1
]}

Xu, Yang ^{[2
]}

Zhu, Ping ^{[2
]}

Shen, Yang ^{[3
]}

Wu, Huihui ^{[1
]}

Ji, Yusi ^{[1
]}

Wen, Xing ^{[1
]}

Zhang, Chen ^{[1
]}

Zhao, Qiong ^{[1
]}

Wang, Yichuan ^{[1
]}

Lu, Jian ^{[1
,3
]}

Guo, Hongwei ^{[1
,3
]}

机构：

[1] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[2] Peking Univ, Sch Life Sci, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China

[3] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

来源：

SCIENCE | 2015年 / 348卷 / 6230期

基金：

中国国家自然科学基金;

关键词：

ETHYLENE GAS; TRANSCRIPTION; ETHYLENE-INSENSITIVE3; BIOGENESIS; RESPONSES; TURNOVER;

D O I：

10.1126/science.aaa2618

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plant immunity against foreign gene invasion takes advantage of posttranscriptional gene silencing (PTGS). How plants elaborately avert inappropriate PTGS of endogenous coding genes remains unclear. We demonstrate in Arabidopsis that both 5'-3' and 3'-5' cytoplasmic RNA decay pathways act as repressors of transgene and endogenous PTGS. Disruption of bidirectional cytoplasmic RNA decay leads to pleiotropic developmental defects and drastic transcriptomic alterations, which are substantially rescued by PTGS mutants. Upon dysfunction of bidirectional RNA decay, a large number of 21- to 22-nucleotide endogenous small interfering RNAs are produced from coding transcripts, including multiple microRNA targets, which could interfere with their cognate gene expression and functions. This study highlights the risk of unwanted PTGS and identifies cytoplasmic RNA decay pathways as safeguards of plant transcriptome and development.

引用

页码：120 / 123

页数：4

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