AT oligonucleotides inducing B lymphocyte activation exist in probiotic Lactobacillus gasseri

被引:37
作者
Kitazawa, H
Ueha, S
Itoh, S
Watanabe, H
Konno, K
Kawai, Y
Saito, T
Itoh, T
Yamaguchi, T
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Prod Chem, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] Tohoku Univ, Grad Sch Agr Sci, Lab Funct Morphol, Aoba Ku, Sendai, Miyagi 9818555, Japan
关键词
AT oligonucleotide; B lymphocyte; L; gasseri; probiotic LAB; mitogenic activity;
D O I
10.1016/S0168-1605(00)00500-6
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
This study determined oligonucleotide sequences of mitogenic DNA derived from lactic acid bacteria (LAB). The chromosomal DNA, which was purified from 12 out of 16 strains of Lactobacillus acidophilus group LAB, induced proliferation of splenic lymphocytes. When DNA from L. gasseri JCM1131(T) was cloned and amplified using PCR, the mitogenic activities of B lymphocytes were significantly increased by 108 of 321 DNA clones. Ten high homologous nucleotide sequences were found as possible DNA sequences of mitogens, and were then chemically synthesized (sOL-LG1 to sOL-LG10). Two nucleotide sequences (sOL-LG7 and sOL-LG10) that consist of only A and T nucleotides (AT oligonucleotides) were characterized as B lymphocyte specific mitogens because they resulted in proliferation of B lymphocytes but not of T lymphocytes, sOL-LG7 preferentially bound to large B lymphocytes and enhanced the expression of the CD86 antigen more than the CD69 antigen on B lymphocytes. The findings show that mitogenic AT oligonucleotides are likely to restrict pre-activated subsets of B lymphocytes. This study demonstrated that novel AT oligonucleotides triggering B lymphocyte mitogenic responses exist in the nucleoids of L. gasseri and proposed chat they have potential as applicants for the production of new functional foods, "Bio-Defense Foods". (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:149 / 162
页数:14
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