ER Adaptor SCAP Translocates and Recruits IRF3 to Perinuclear Microsome Induced by Cytosolic Microbial DNAs

被引：76

作者：

Chen, Wei ^{[1
,2
]}

Li, Senlin ^{[1
]}

Yu, Huansha ^{[1
]}

Liu, Xing ^{[1
]}

Huang, Lulu ^{[1
]}

Wang, Qiang ^{[1
]}

Liu, Heng ^{[1
]}

Cui, Ye ^{[1
]}

Tang, Yijun ^{[1
]}

Zhang, Peng ^{[3
]}

Wang, Chen ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Excellence Mol Cell Sci,State Key Lab Cell Bi, Innovat Ctr Cell Signaling Network,Inst Biochem &, Shanghai, Peoples R China

[2] Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Key Lab Med Reprogramming Technol, Shenzhen, Peoples R China

[3] Tongji Univ, Sch Med, Shanghai Pulm Hosp, Dept Thorac Surg, Shanghai 200092, Peoples R China

来源：

PLOS PATHOGENS | 2016年 / 12卷 / 02期

基金：

中国国家自然科学基金;

关键词：

INNATE IMMUNE SENSOR; CYCLIC GMP-AMP; I INTERFERON; SIGNAL-TRANSDUCTION; RECOGNITION; PROTEIN; SREBP; IDENTIFICATION; ACTIVATION; RECEPTORS;

D O I：

10.1371/journal.ppat.1005462

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Stimulator of interferon genes (STING, also known as MITA, ERIS or MPYS) induces the activation of TBK1 kinase and IRF3 transcription factor, upon sensing of microbial DNAs. How IRF3 is recruited onto the STING signalosome remains unknown. We report here that silencing of the ER adaptor SCAP markedly impairs the IRF3-responsive gene expression induced by STING. Scap knockdown mice are more susceptible to HSV-1 infection. Interestingly, SCAP translocates from ER, via Golgi, to perinuclear microsome in a STING-dependent manner. Mechanistically, the N-terminal transmembrane domain of SCAP interacts with STING, and the C-terminal cytosolic domain of SCAP binds to IRF3, thus recruiting IRF3 onto STING signalosome. Mis-localization of SCAP abolishes its antiviral function. Collectively, this study characterizes SCAP as an essential adaptor in the STING signaling pathway, uncovering a critical missing link in DNAs-triggered host antiviral responses.

引用

页数：22

共 43 条

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