Cellular micropatterns on biocompatible materials

被引:223
作者
Folch, A
Toner, M
机构
[1] Harvard Univ, Sch Med, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Ctr Engn Med, Boston, MA 02114 USA
[3] Massachusetts Gen Hosp, Surg Serv, Boston, MA 02114 USA
[4] Shriners Burns Hosp, Boston, MA 02114 USA
关键词
D O I
10.1021/bp980037b
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We present a method to produce micropatterns of cells on tissue culture substrates. A network of deep elastomeric microchannels defining the desired pattern is sealed onto the surface of interest, and a protein template is created by injecting sub-milliliter quantities of protein solution into the microchannels. Protein adsorbs only on the areas that were exposed to the microflow. After the channels are flushed and the elastomer is removed, cells attach only on the protein template. Micropatterns of collagen or fibronectin were used to selectively adhere cells on various biomedical polymers and on heterogeneous or microtextured substrates. Since the bare substrate areas remain apt for seeding other, more adhesive cell types such as fibroblasts, we were able to create micropatterned co-cultures. Our method allows for inexpensive patterning of a rich assortment of biomolecules, cells, and surfaces under physiological conditions.
引用
收藏
页码:388 / 392
页数:5
相关论文
共 44 条
[1]   CRITICAL ISSUES IN APPLICATIONS OF SELF-ASSEMBLED MONOLAYERS [J].
ALLARA, DL .
BIOSENSORS & BIOELECTRONICS, 1995, 10 (9-10) :771-783
[2]   Microfabrication of hepatocyte/fibroblast co-cultures: Role of homotypic cell interactions [J].
Bhatia, SN ;
Balis, UJ ;
Yarmush, ML ;
Toner, M .
BIOTECHNOLOGY PROGRESS, 1998, 14 (03) :378-387
[3]  
Bhatia SN, 1997, J BIOMED MATER RES, V34, P189, DOI 10.1002/(SICI)1097-4636(199702)34:2<189::AID-JBM8>3.0.CO
[4]  
2-M
[5]   PRINCIPLES OF CELL MOTILITY - DIRECTION OF CELL MOVEMENT AND CANCER INVASION [J].
CARTER, SB .
NATURE, 1965, 208 (5016) :1183-&
[7]   Geometric control of cell life and death [J].
Chen, CS ;
Mrksich, M ;
Huang, S ;
Whitesides, GM ;
Ingber, DE .
SCIENCE, 1997, 276 (5317) :1425-1428
[8]  
CLARK P, 1992, J CELL SCI, V103, P287
[9]  
CURTIS ASG, 1986, J CELL SCI, V86, P9
[10]   Patterned delivery of immunoglobulins to surfaces using microfluidic networks [J].
Delamarche, E ;
Bernard, A ;
Schmid, H ;
Michel, B ;
Biebuyck, H .
SCIENCE, 1997, 276 (5313) :779-781