TNA synthesis by DNA polymerases

被引：121

作者：

Chaput, JC

Szostak, JW ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, Howard Hughes Med Inst, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Dept Biol Mol, Boston, MA 02114 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 31期

关键词：

D O I：

10.1021/ja035917n

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Threose nucleic acid (TNA), which has a repeat unit one atom shorter than that of DNA, is capable of Watson-Crick base pairing with DNA, RNA, and TNA. Because of its chemical simplicity, TNA is considered to be a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for TNA synthesis activity on a DNA template. We wish to report that several polymerases show surprisingly good ability to synthesize TNA using α-l-threofuranosyl thymidine-3′-triphosphate as a substrate. Copyright © 2003 American Chemical Society.

引用

页码：9274 / 9275

页数：2

共 24 条

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