A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens

被引:27
作者
Whiley, DM
Buda, PP
Freeman, K
Pattle, NI
Bates, J
Sloots, TP
机构
[1] Royal Childrens Hosp & Hlth Serv Dist, Sir Albert Sakzewski Virus Res Ctr, Clin Virol Res Unit, Herston, Qld 4029, Australia
[2] Royal Darwin Hosp, Dept Pathol, Microbiol Lab, Darwin, NT 0811, Australia
[3] Univ Queensland, Dept Paediat & Child Hlth, Brisbane, Qld 4029, Australia
关键词
Neisseria gonorrhoeae; real-time PCR; LightCycler;
D O I
10.1016/j.diagmicrobio.2004.12.011
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
A Neisseria gonorrhoeae LightCycler (NGpapLC) assay targeting the porA pseudogene was compared with bacterial culture for detection of N.gonorrhoeae in 636 clinical specimens (216 cervical, 185 urethral, 196 throat, and 39 rectal swab specimens). The specificity of the NGpapLC assay was further investigated by testing a bacterial reference panel comprising several Neisseria species. Overall, 19 (3.0%) specimens were positive and 613 (96.4%) specimens were negative by both methods. Four (0.6%) specimens were positive by the NGpapLC assay only. For the cervical and urethral swabs, the NGpapLC provided 100% sensitivity and 100% specificity compared with bacterial culture. Following discrepant analysis, the clinical sensitivity and specificity of the NGpapLC for throat and rectal swabs was also 100%. For the bacterial panel, only N. gonorrhoeae isolates provided positive results. The results show the NGpapLC assay is suitable for use on a range of clinical specimens and could improve detection of pharyngeal N. gonorrhoeae. (c) 2005 Elsevier Inc. All rights reserved.
引用
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页码:1 / 5
页数:5
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