Identification of novel urinary metabolites of the lipid peroxidation product 4-hydroxy-2-nonenal in rats

被引：34

作者：

Alary, J ^{[1
]}

Debrauwer, L ^{[1
]}

Fernandez, Y ^{[1
]}

Paris, A ^{[1
]}

Cravedi, JP ^{[1
]}

Dolo, L ^{[1
]}

Rao, D ^{[1
]}

Bories, G ^{[1
]}

机构：

[1] INRA, Lab Xenobiot, F-31931 Toulouse, France

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 1998年 / 11卷 / 11期

关键词：

D O I：

10.1021/tx980068g

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Following iv administration of 4-hydroxy-2-nonenal (HNE) and [4-H-3]HNE to rats, 15 polar urinary metabolites accounting for about 50% of the urinary radioactivity were separated by HPLC, Among them, eight major compounds and tritiated water were quantified. The metabolites were unequivocally characterized using GC/MS and ESI/MS/MS/MS. Most of "HNE polar metabolites" originate from omega-oxidation of 4-hydroxy-2-nonenoic acid(HNA): 9-hydroxy-HNA, its mercapturic acid conjugate, and two diastereoisomers of the corresponding lactone. The oxidation of 9-hydroxy-HNA by alcohol and aldehyde dehydrogenases leads to the excretion of 9-carboxy-HNA and of the corresponding lactone mercapturic acid conjugate. 1,4-Dihydroxy-2-nonene (DHN) originating from the reduction of HNE by alcohol dehydrogenase was to a lesser extent omega-hydroxylated, leading to 9-hydroxy-DHN which was excreted as a mercapturic acid conjugate (two diastereoisomers).

引用

页码：1368 / 1376

页数：9

共 17 条

[1] MERCAPTURIC ACID CONJUGATES AS URINARY END METABOLITES OF THE LIPID-PEROXIDATION PRODUCT 4-HYDROXY-2-NONENAL IN THE RAT [J].