Genomic alterations in oral squamous cell carcinoma cell lines detected by two-dimensional gel analysis

被引：4

作者：

Yamamoto, K

Konishi, N ^{[1
]}

Inui, T

Kitahori, Y

Hiasa, Y

Kirita, T

Sugimura, M

机构：

[1] Nara Med Univ, Dept Pathol 2, Nara 6348521, Japan

[2] Nara Med Univ, Dept Oral & Maxillofacial Surg, Nara 6348521, Japan

来源：

ORAL ONCOLOGY | 1998年 / 34卷 / 06期

关键词：

oral squamous cell carcinoma; cell line; genome; RLGS;

D O I：

10.1016/S1368-8375(98)00030-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To initially analyze the genomic abnormalities in human oral squamous cell carcinoma, DNA extracted from each of four oral carcinoma cell lines (Ca9-22, HO-1-u-1, HSC-2, KB) was examined using restriction landmark genomic scanning (RLGS), a method especially conducive to detection of amplifications and rearrangements of genomic DNA. Isolated cell line and normal oral epithial DNAs were sequentially cleaved with specific restriction enzymes, radiolabelled and separated in two-dimensional gel electrophoreses. Thirteen distinct fragments were commonly amplified in the oral cancer cell lines, six of which were evident in all samples. These results suggest genetic alterations characteristic of oral squamous cell carcinogenesis. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：508 / 512

页数：5

共 22 条

[1]

ANKITA MP, 1996, ONCOGENE, V12, P31

[2]

Bockmuhl U, 1996, CANCER RES, V56, P5325

[3]

Califano J, 1996, CANCER RES, V56, P2488

[4] ROLES OF P53 MUTATION IN CELL-LINE ESTABLISHMENT AND IDENTIFICATION OF THE MINIMUM TRANSACTIVATION AND TRANSFORM SUPPRESSION DOMAINS [J].