Effect of sulfated astragalus polysaccharide on cellular infectivity of infectious bursal disease virus

被引：101

作者：

Huang, Xiaoyan ^{[1
]}

Wang, Deyun ^{[1
]}

Hu, Yuanhang ^{[1
]}

Lu, Yu ^{[1
]}

Guo, Zhenhuan ^{[1
]}

Kong, Xiangfeng ^{[1
]}

Sun, Junfing ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 42卷 / 02期

基金：

中国国家自然科学基金;

关键词：

astragalus polysaccharide; sulfated modification; bursal disease virus; cellular infectivity;

D O I：

10.1016/j.ijbiomac.2007.10.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Four kinds of astragalus polysaccharides (APSs), APS(t), APS(40), APS(50) and APS(60), were extracted by water decoction and one-step or stepwise ethanol precipitation methods, and modified by chlorosulfonic acid-pyridine method to obtain four sulfated APSs (sAPSs) (sAPS(t), sAPS(40), sAPS(50), sAPS(60)), respectively. The effects of four sAPSs on cellular infectivity of bursal disease virus (IBDV) were compared by MTT method taking non-modified APSt as control. The results showed that modified sAPSs inhibited IBDV to infect CEF significantly in comparison with non-modified APSt, which indicated that sulfated modification could enhance the antiviral activity of the APS, by which it would be expected to develop a new-type antiviral drug. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：166 / 171

页数：6

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