Evidence for control of nitrogen metabolism by a START-dependent mechanism in Saccharomyces cerevisiae

被引:3
作者
Bryan, BA [1 ]
McGrew, E [1 ]
Lu, Y [1 ]
Polymenis, M [1 ]
机构
[1] Texas A&M Univ, Dept Biochem & Biophys, College Stn, TX 77843 USA
关键词
Glt1p; Jem1p; CLN; START; chemostat;
D O I
10.1007/s00438-003-0957-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is generally thought that cell growth and metabolism regulate cell division and not vice versa. Here, we examined Saccharomyces cerevisiae cells growing under conditions of continuous culture in a chemostat. We found that loss of G1 cyclins, or inactivation of the cyclin-dependent kinase Cdc28p, reduced the activity of glutamate synthase (Glt1p), a key enzyme in nitrogen assimilation. We also present evidence indicating that the G1 cyclin-dependent control of Glt1p may involve Jem1p, a DnaJ-type chaperone. Our results suggest that completion of START may be linked to nitrogen metabolism.
引用
收藏
页码:72 / 81
页数:10
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