Protection of baculovirus-vectors against complement-mediated inactivation by recombinant soluble complement receptor type 1

被引：31

作者：

Hofmann, C

Hüser, A

Lehnert, W

Strauss, M

机构：

[1] HepaVec AG Gentherapie, D-13122 Berlin, Germany

[2] Humboldt Univ, Max Delbruck Ctr Mol Med, D-13122 Berlin, Germany

[3] Danish Canc Soc, Div Canc Biol, DK-2100 Copenhagen, Denmark

来源：

BIOLOGICAL CHEMISTRY | 1999年 / 380卷 / 03期

关键词：

baculovirus vectors; hepatocytes; liver gene transfer; protection; soluble complement receptor;

D O I：

10.1515/BC.1999.052

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Baculovirus-based vectors are efficient means for gene transfer into hepatocytes in vitro. However, gene transfer in vivo is hampered by inactivation of baculovirus by the complement system. In this study, we demonstrate protection of baculovirus vectors against complement-mediated inactivation through recombinant soluble complement receptor type 1 (sCR1). Blocking of only the alternative complement pathway by a mutant of sCR1 did not result in baculovirus survival in human serum. The data suggest the use of sCR1 as a potent drug to facilitate baculovirus-mediated gene transfer into hepatocytes in vivo.

引用

页码：393 / 395

页数：3

共 20 条

[1] Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein [J].