Purification and characterization of a soluble polyurethane degrading enzyme from Comamonas acidovorans

被引：39

作者：

Allen, AB

Hilliard, NP

Howard, GT ^{[1
]}

机构：

[1] SE Louisiana Univ, Dept Biol Sci, Hammond, LA 70402 USA

[2] SE Louisiana Univ, Dept Chem & Phys, Hammond, LA 70402 USA

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 1999年 / 43卷 / 1-2期

关键词：

comamonas acidovorans; polyurethane degradation; enzyme purification;

D O I：

10.1016/S0964-8305(98)00066-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A soluble esterase involved in the biodegradation of polyester polyurethane (PU) was purified to apparent electrophoretic homogeneity in high yield, similar to 83%. The enzyme displayed a single band on both non-denaturing (ND-) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with an apparent molecular mass of 42 kDa. Using rho-nitrophenylacetate as the substrate, the enzyme displayed steady-state kinetic parameters K-m and V-max of 51.5 mM and 180 U mg(-1) respectively. Esterase activity was thermally stable and could be inhibited with phenylmethylsulfonylfluoride (PMSF) and soybean trypsin inhibitor (STI). (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：37 / 41

页数：5

共 19 条

[1]

Akutsu Y, 1998, APPL ENVIRON MICROB, V64, P62

[2] RAPID ASSESSMENT OF THE MICROBIAL DETERIORATION OF POLYURETHANES [J].