Characterisation of genes involved in biosynthesis of coronafacic acid, the polyketide component of the phytotoxin coronatine

被引：28

作者：

Penfold, CN

Bender, CL

Turner, JG

机构：

[1] UNIV E ANGLIA, SCH BIOL SCI, MOL BIOL SECTOR, NORWICH NR4 7TJ, NORFOLK, ENGLAND

[2] OKLAHOMA STATE UNIV, DEPT PLANT PATHOL, STILLWATER, OK 74078 USA

来源：

GENE | 1996年 / 183卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

Pseudomonas syringae pv glycinea; coronafacic acid; acyl carrier protein; acyltransferase; beta-ketoacylsynthase; dehydrase; type II polyketide synthase;

D O I：

10.1016/S0378-1119(96)00550-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Coronafacic acid (CFA) is the polyketide component of coronatine (COR), a phytotoxin produced by the plant pathogen, Pseudomonas syringae. In the present study we have determined the nucleotide sequence of a 3.92-kb DNA fragment involved in CFA biosynthesis. Analysis of the sequence revealed four complete open reading frames (ORFs) designated cfa1 to cfa4 and one incomplete ORF (cfa5), all transcribed in the same direction. The predicted translation products of cfa1, cfa2 and cfa3 showed relatedness to acyl carrier proteins, fatty acid dehydrases and beta-ketoacylsynthases, respectively, which are required for polyketide synthesis. cfa1 was subcloned, its sequence was confirmed, and it was overexpressed in E. coli to yield a peptide with an apparent molecular mass of 6 kDa.

引用

页码：167 / 173

页数：7

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