Arabinogalactan for hepatic drug delivery .3. Development of an immunoassay for larch arabinogalactan and its use in the detection of larch arabinogalactan in rat blood

被引：13

作者：

Groman, EV

Gou, DM

机构：

[1] Advanced Magnetics, Inc., Cambridge, MA 02138

来源：

CARBOHYDRATE RESEARCH | 1997年 / 301卷 / 1-2期

关键词：

arabinogalactan; immunoassay; pharmacokinetics;

D O I：

10.1016/S0008-6215(97)00077-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

We describe a sensitive and convenient immunoassay for larch arabinogalactan and demonstrate its specificity for larch arabinogalactan. Anti-larch arabinogalactan antiserum is about 10(4) and 10(6) times more selective for detecting larch arabinogalactan than RCA lectin and rat-liver asialoglycoprotein receptor, respectively. Anti-larch arabinogalactan antiserum binds to branch terminal disaccharides consisting of the terminal beta-D-galactosyl residue and the penultimate branch (1 --> 6)-beta-D-galactosyl residue. It does not bind L-arabinose. The sensitivity of the assay for larch arabinogalactan is less than 0.1 mu g/mL. The application of the assay for measuring arabinegalactan pharmacokinetics in rat blood is illustrated. (C) 1997 Elsevier Science Ltd.

引用

页码：69 / 76

页数：8

共 21 条

[1]

STUDIES ON ARABOGALACTANS .4. A METHYLATION STUDY OF ARABOGALACTAN-B FROM LARIX-OCCIDENTALIS NUTT [J].