The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions

被引：102

作者：

Nolan, JP

Sklar, LA

机构：

[1] Los Alamos Natl Lab, Natl Flow Cytometry Resource & Life Sci Div, Los Alamos, NM 87545 USA

[2] Univ New Mexico, Ctr Canc, Albuquerque, NM 87131 USA

[3] Univ New Mexico, Dept Pathol, Albuquerque, NM 87131 USA

来源：

NATURE BIOTECHNOLOGY | 1998年 / 16卷 / 07期

关键词：

technology; biosensor; fluorescence; fusion proteins;

D O I：

10.1038/nbt0798-633

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The analysis of macromolecular interactions is an essential element of biomedical research. Flow cytometry is uniquely capable of making sensitive and quantitative measurements of molecular interactions. These measurements can be made in real time with subsecond kinetic resolution using purified biomolecules or living cells. Combined with automated sample handling, these features make flow cytometry a versatile and robust technology for the analysis of molecular interactions.

引用

页码：633 / 638

页数：6

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