In vivo resolution of conflicting in vitro results: Synthesis of biotin from dethiobiotin does not require pyridoxal phosphate

被引：7

作者：

Abdel-Hamid, Ahmed M.

Cronan, John E. ^{[1
]}

机构：

[1] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

来源：

CHEMISTRY & BIOLOGY | 2007年 / 14卷 / 11期

关键词：

D O I：

10.1016/j.chembiol.2007.10.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The source of the biotin sulfur atom remains a contested point in studies of biotin synthase (BioB) in vitro. Recent reports that BioB has an intrinsic pyridoxal phosphate (PLP)-dependent cysteine desulfurase activity were tested by depleting Escherichia coli cells of PLP. The BioB-dependent conversion of dethiobiotin to biotin proceeded in these cells irrespective of the presence or absence of PLP.

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收藏

页码：1215 / 1220

页数：6

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