Gene transfer vectors derived from equine infectious anemia virus

被引:171
作者
Olsen, JC [1 ]
机构
[1] Univ N Carolina, Dept Med, Cyst Fibrosis Pulm Res & Treatment Ctr, Chapel Hill, NC 27514 USA
关键词
lentiviral vector; equine infectious anemia virus; aphidicolin;
D O I
10.1038/sj.gt.3300768
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Equine infectious anemia virus (EIAV) is a lentivirus in the retrovirus family of viruses. Replication-defective EIAV vectors have been constructed that encode bacterial puromycin-N-acetyl transferase and E. coli beta-galactosidase. These vectors could be prepared with titers greater than 10(5 )infectious units/ml and were able to act as vehicles to carry genes into cultured human cells. In addition, stable helper cell lines were created by modifying human 293 cells to express EIAV proteins. Unlike retroviral vectors based on murine leukemia virus, EIAV lentiviral vectors transduce nondividing (aphidicolin-arrested) cells. These properties make EIAV vectors promising gene transfer vehicles.
引用
收藏
页码:1481 / 1487
页数:7
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