Multicenter Evaluation of a New DNA Microarray for Rapid Detection of Clinically Relevant bla Genes from β-Lactam-Resistant Gram-Negative Bacteria

被引：33

作者：

Bogaerts, Pierre ^{[1
]}

Hujer, Andrea M. ^{[2
,3
]}

Naas, Thierry ^{[4
]}

de Castro, Roberta Rezende ^{[1
]}

Endimiani, Andrea ^{[2
,3
]}

Nordmann, Patrice ^{[4
]}

Glupczynski, Youri ^{[1
]}

Bonomo, Robert A. ^{[2
,3
]}

机构：

[1] Clin Univ UCL Mt Godinne, Bacteriol Lab, B-5530 Yvoir, Belgium

[2] Case Western Reserve Univ, Sch Med, Dept Med, Cleveland, OH 44106 USA

[3] Vet Affairs Med Ctr, Louis Stokes Cleveland Dept, Cleveland, OH USA

[4] Hop Bicetre, AP HP, Serv Bacteriol Virol,Fac Med Paris, INSERM Emerging Resistance Antibiot U914, F-94275 Le Kremlin Bicetre, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 09期

基金：

美国国家卫生研究院;

关键词：

ESCHERICHIA-COLI; CTX-M; BELGIAN HOSPITALS; CARBAPENEMASES; INFECTIONS; EMERGENCE; SHV; TEM;

D O I：

10.1128/AAC.00353-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new commercial low-density microarray which identifies common extended-spectrum beta-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.

引用

页码：4457 / 4460

页数：4

共 17 条

[1] [Anonymous], 2010, ANTIMICROB AGENTS CH, DOI DOI 10.1128/AAC.01009-09
[2] Emergence of clonally related Klebsiella pneumoniae isolates of sequence type 258 producing KPC-2 carbapenemase in Belgium
Bogaerts, P.
Montesinos, I.
Rodriguez-Villalobos, H.
Blairon, L.
Deplano, A.
Glupczynski, Y.
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (02) : 361 - 362
[3] Emergence of extended-spectrum-AmpC-expressing Escherichia coli isolates in Belgian hospitals
Bogaerts, Pierre
Rodriguez-Villalobos, Hector
Laurent, Christine
Deplano, Ariane
Struelens, Marc J.
Glupczynski, Youri
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 63 (05) : 1073 - 1075
[4] Bench-to-bedside review: The role of β-lactamases in antibiotic-resistant Gram-negative infections
Bush, Karen
[J]. CRITICAL CARE, 2010, 14 (03)
[5] Evaluation of a Commercial Microarray System for Detection of SHV-, TEM-, CTX-M-, and KPC-Type β-Lactamase Genes in Gram-Negative Isolates
Endimiani, Andrea
Hujer, Andrea M.
Hujer, Kristine M.
Gatta, Julian A.
Schriver, Andrew C.
Jacobs, Michael R.
Rice, Louis B.
Bonomo, Robert A.
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (07) : 2618 - 2622
[6] AmpC β-Lactamases
Jacoby, George A.
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2009, 22 (01) : 161 - +
[7] Has the era of untreatable infections arrived?
Livermore, David M.
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2009, 64 (04) : 29 - 36
[8] Phenotypic and Biochemical Comparison of the Carbapenem-Hydrolyzing Activities of Five Plasmid-Borne AmpC β-Lactamases
Mammeri, Hedi
Guillon, Helene
Eb, Francois
Nordmann, Patrice
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (11) : 4556 - 4560
[9] Minor extended-spectrum β-lactamases
Naas, T.
Poirel, L.
Nordmann, P.
[J]. CLINICAL MICROBIOLOGY AND INFECTION, 2008, 14 : 42 - 52
[10] Evaluation of a DNA Microarray, the Check-Points ESBL/KPC Array, for Rapid Detection of TEM, SHV, and CTX-M Extended-Spectrum β-Lactamases and KPC Carbapenemases
Naas, T.
Cuzon, G.
Truong, H.
Bernabeu, S.
Nordmann, P.
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (08) : 3086 - 3092

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