Cadherin-mediated cell interaction regulates germ cell determination in mice

被引：59

作者：

Okamura, D

Kimura, T

Nakano, T

Matsui, Y

机构：

[1] Osaka Med Ctr Maternal & Child Hlth, Res Inst, Dept Mol Embryol, Izumi, Osaka 5941101, Japan

[2] Osaka Univ, Grad Sch Med, Suita, Osaka, Japan

[3] Osaka Univ, Dept Mol Cell Biol, Microbial Dis Res Inst, Suita, Osaka, Japan

来源：

DEVELOPMENT | 2003年 / 130卷 / 26期

关键词：

primordial germ cell; E-cadherin; cell interaction; cell fate;

D O I：

10.1242/dev.00870

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The germ cell lineage segregates from the somatic cell lineages in early embryos. Germ cell determination in mice is not regulated by maternally inherited germplasm, but is initiated within the embryo during gastrulation. However, the mechanisms of germ cell specification in mice remain unknown. We located precursors to primordial germ cells (PGCs) within early embryos, and show here that cell-cell interaction among these precursors is required for germ cell specification. We found that the expression of a calcium-dependent cell adhesion molecule, E-cadherin, is restricted to the proximal region of extra-embryonic mesoderm that contains PGC precursors, and that blocking the functions of E-cadherin with an antibody inhibits PGC formation in vitro. These results showed that E-cadherin-mediated cell-cell interaction among cells containing PGC precursors is essential to directing such cells to the germ cell fate.

引用

页码：6423 / 6430

页数：8

共 38 条

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