BSA Adsorption on Differently Charged Polystyrene Nanoparticles using Isothermal Titration Calorimetry and the Influence on Cellular Uptake

被引：136

作者：

Baier, Grit ^{[1
]}

Costa, Cristiane ^{[2
]}

Zeller, Anke ^{[1
]}

Baumann, Daniela ^{[1
]}

Sayer, Claudia ^{[2
]}

Araujo, Pedro H. H. ^{[2
]}

Mailaender, Volker ^{[1
,3
]}

Musyanovych, Anna ^{[1
]}

Landfester, Katharina ^{[1
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[2] Univ Fed Santa Catarina, Dept Engn Quim & Alimentos, CTC, BR-88040970 Florianopolis, SC, Brazil

[3] Johannes Gutenberg Univ Mainz, Univ Med, Med Clin 3, D-55131 Mainz, Germany

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 05期

关键词：

bovine serum albumin; cellular uptake; isothermal titration calorimetry; polyelectrolyte; polystyrene nanoparticles; BOVINE SERUM-ALBUMIN; PROTEIN ADSORPTION; MINIEMULSION POLYMERIZATION; PARTICLES; CARBOXYL; CELLS; SIZE;

D O I：

10.1002/mabi.201000395

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the particle, was studied using ITC. Different thermodynamic data such as enthalpy changes upon binding and stoichiometry of the systems were determined and discussed. The degree of surface coverage with BSA was calculated using the thermodynamic data. The cellular uptake of particles before and after BSA adsorption was studied using HeLa cells in the presence and absence of supplemented FCS in the cell culture medium.

引用

页码：628 / 638

页数：11

共 32 条

[1]

Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy [J].