Distribution of genes for synthesis of trehalose and mannosylglycerate in Thermus spp. and direct correlation of these genes with halotolerance

被引:31
作者
Alarico, S
Empadinhas, N
Simoes, C
Silva, Z
Henne, A
Mingote, A
Santos, H
da Costa, MS [1 ]
机构
[1] Univ Coimbra, Dept Bioquim, P-3004517 Coimbra, Portugal
[2] Univ Coimbra, Ctr Neurociencias & Biol Celular, Dept Zool, P-3004517 Coimbra, Portugal
[3] Inst Mikrobiol & Genet, Gottingen Genom Lab, D-37077 Gottingen, Germany
[4] Univ Nova Lisboa, Inst Tecnol Quim & Biol, P-2780156 Oeiras, Portugal
关键词
D O I
10.1128/AEM.71.5.2460-2466.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this study we correlate the presence of genes leading to the synthesis of trehalose and mannosylglycerate (MG) in 17 strains of the genus Thermus with the ability of the strains to grow and accumulate these compatible solutes in a defined medium containing NaCl. The two sets of genes, namely, otsA/otsB for the synthesis of trehalose and mpgS/mpgP for the synthesis of MG, were necessary for the growth of Thermus thermophilus in a defined medium containing up to 6% NaCl. Strains lacking a complete otsA gene did not grow in defined medium containing > 2% NaCl. One strain of T. thermophilus lacking the genes for the synthesis of MG did not grow in a medium with > 1% NaCl. We did not identify any of these genes in the type strains of the other seven species of Thermus, and none of those strains grew in defined medium with 1% NaCl. The results strongly indicate that the combined accumulation of trehalose and MG is required for optimal osmotic adjustment.
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收藏
页码:2460 / 2466
页数:7
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