Determination of domain structure of proteins from X-ray solution scattering

被引：1159

作者：

Svergun, DI

Petoukhov, MV

Koch, MHJ

机构：

[1] DESY, EMBL, Hamburg Outstn, D-22603 Hamburg, Germany

[2] Russian Acad Sci, Inst Crystallog, Moscow 117333, Russia

[3] Moscow MV Lomonosov State Univ, Dept Phys, Moscow 117234, Russia

来源：

BIOPHYSICAL JOURNAL | 2001年 / 80卷 / 06期

关键词：

D O I：

10.1016/S0006-3495(01)76260-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An ab initio method for building structural models of proteins from x-ray solution scattering data is presented. Simulated annealing is employed to find a chain-compatible spatial distribution of dummy residues which fits the experimental scattering pattern up to a resolution of 0.5 nm. The efficiency of the method is illustrated by the ab initio reconstruction of models of several proteins, with known and unknown crystal structure, from experimental scattering data. The new method substantially improves the resolution and reliability of models derived from scattering data and makes solution scattering a useful technique in large-scale structural characterization of proteins.

引用

收藏

页码：2946 / 2953

页数：8

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