Magnetizable antibody-like proteins

被引:13
作者
Dehal, Prabhjyot Kaur [1 ]
Livingston, Christine Frances [1 ]
Dunn, Claire Geekie [1 ]
Buick, Richard [2 ]
Luxton, Richard [3 ]
Pritchard, David John [1 ]
机构
[1] Axis Shield Diagnost Ltd, Res & Dev, Dundee DD2 1XA, Scotland
[2] Springbank Ind Estate, FusionAntibodies, Unit 4, Belfast, Antrim, North Ireland
[3] UWE, Inst BioSensing Technol, Bristol, Avon, England
关键词
Analytical biotechnology; Antibody; Ferritin; Paramagnetic; scFv; NUCLEIC-ACIDS; SOLID-PHASE; FERRITIN; BINDING; MAGNETOFERRITIN; NANOPARTICLES; PURIFICATION; SEPARATION; BEADS; MRI;
D O I
10.1002/biot.200900273
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Use of paramagnetic particles to isolate molecules or cells from complex media is well established. Typically, particles are manufactured and coated with a biological molecule that confers specific biorecognition. Incubation of particles with sample and exposure to magnetic fields isolates the species of interest. We have designed, produced and assessed magnetized fusion proteins consisting of the antigen-binding portion of an antibody (single chain variable fraction; scFv) fused to the heavy chain of the iron-binding protein ferritin. The fusion protein subunits expressed in E. coli assemble to form a fusion protein consisting of a ferritin sphere with scFvs on the surface. The fusion proteins were chemically magnetized by introducing a paramagnetic iron core. The resultant fusion protein was shown to be magnetizable and capable of binding target antigens. These "organic" magnetizable particles possess a number of theoretical advantages over traditional inorganic particles.
引用
收藏
页码:596 / 604
页数:9
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