Comprehensive analysis of human endogenous retrovirus transcriptional activity in human tissues with a retrovirus-specific microarray

被引:173
作者
Seifarth, W
Frank, O
Zeilfelder, U
Spiess, B
Greenwood, AD
Hehlmann, R
Leib-Mösch, C
机构
[1] Heidelberg Univ, Fac Clin Med, Med Clin 3, D-68305 Mannheim, Germany
[2] Inst Mol Virol, Natl Res Ctr Environm & Hlth, GSF, Neuherberg, Germany
[3] Tech Univ Munich, Inst Virol, D-8000 Munich, Germany
关键词
D O I
10.1128/JVI.79.1.341-352.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Retrovirus-like sequences account for 8 to 9% of the human genome. Among these sequences, about 8,000 pol-containing proviral elements have been identified to date. As part of our ongoing search for active and possibly disease-relevant human endogenous retroviruses (HERVs), we have recently developed an oligonucleotide-based microarray. The assay allows for both the detection and the identification of most known retroviral reverse transcriptase (RT)-related nucleic acids in biological samples. In the present study, we have investigated the transcriptional activity of representative members of 20 HERV families in 19 different normal human tissues. Qualitative evaluation of chip hybridization signals and quantitative analysis by real-time RT-PCR revealed distinct HERV activity in the human tissues under investigation, suggesting that HERV elements are active in human cells in a tissue-specific manner. Most active members of HERV families were found in mRNA prepared from skin, thyroid gland, placenta, and tissues of reproductive organs. In contrast, only few active HERVs were detectable in muscle cells. Human tissues that lack HERV transcription could not be found, confirming that human endogenous retroviruses are permanent components of the human transcriptome. Distinct activity patterns may reflect the characteristics of the regulatory machinery in these cells, e.g., cell type-dependent occurrence of transcriptional regulatory factors.
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收藏
页码:341 / 352
页数:12
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