Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein

被引：406

作者：

Seo, SB

McNamara, P

Heo, S

Turner, A

Lane, WS

Chakravarti, D ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Pharmacol, Philadelphia, PA 19104 USA

[2] Harvard Univ, Harvard Microchem & Prot Facil, Cambridge, MA 02138 USA

来源：

CELL | 2001年 / 104卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(01)00196-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetylation of histones by p300/CBP and PCAF is considered to be a critical step in transcriptional regulation. In order to understand the role of cellular activities that modulate histone acetylation and transcription, we have purified and characterized a multiprotein cellular complex that potently inhibits the histone acetyltransferase activity of p300/CBP and PCAF. We have mapped a novel acetyltransferase-inhibitory domain of this INHAT (inhibitor of acetyltransferases) complex that binds to histones and masks them from being acetyltransferase substrates. Endogenous INHAT subunits, which include the Set/TAF-I beta oncoprotein, associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. We propose that histone masking by INHAT plays a regulatory role in chromatin modification and serves as a novel mechanism of transcriptional regulation.

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页码：119 / 130

页数：12

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