Immune responses to heat shock protein in Porphyromonas gingivalis-infected periodontitis and atherosclerosis patients

被引:36
作者
Chung, SW
Kang, HS
Park, HR
Kim, SJ
Kim, SJ
Choi, JI
机构
[1] Pusan Natl Univ, Sch Dent, Dept Periodontol, Seo Ku, Pusan 602739, South Korea
[2] Pusan Natl Univ, Sch Dent, Dept Oral Pathol, Pusan 609735, South Korea
[3] Pusan Natl Univ, Coll Nat Sci, Dept Mol Biol, Pusan 609735, South Korea
[4] Pusan Natl Univ, Sch Med, Dept Thorac & Cardiovasc Surg, Pusan 609735, South Korea
关键词
Porphyromonas gingivalis; heat shock protein; periodontitis; atherosclerosis;
D O I
10.1034/j.1600-0765.2003.00664.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: It has been widely thought that heat shock protein might be involved in autoimmune disease mechanisms in humans. Objectives: The present study was performed to evaluate the recognition of Porphyromonas gingivalis heat shock protein 60 (hsp60) and human hsp60 by immune sera in P. gingivalis-infected periodontitis and atherosclerosis patients. Materials and method. Mononuclear cells from atheroma lesions were stimulated with P. gingivalis hsp and sera from periodontitis or atherosclerosis patients were subjected to Western immunoblotting to P. gingivalis hsp or human hsp, respectively. Results: Western immunoblot analysis demonstrated the dual reactivity of anti-P. gingivalis antisera with P. gingivalis hsp and human hsp. We could also establish P. gingivalis hsp-specific T cell lines from the atheroma lesions, a mixture of CD4(+) and CD8(+) cells producing the cytokines characteristic of both Th1 and Th2 subsets. Conclusion: These observations suggest the modulating effect of P. gingivalis hsp60 in the immunopathogenesis of periodontitis and atherosclerosis.
引用
收藏
页码:388 / 393
页数:6
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