Increased thermostability of cross-linked enzyme crystals of subtilisin in organic solvents

被引：23

作者：

Noritomi, H ^{[1
]}

Koyama, K ^{[1
]}

Kato, S ^{[1
]}

Nagahama, K ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Grad Sch Engn, Dept Appl Chem, Tokyo 1920397, Japan

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 06期

关键词：

D O I：

10.1023/A:1008863407130

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cross-linked enzyme crystals(CLECs) of subtilisin display the improved thermostability in organic solvents, compared to free subtilisin. CLECs are more stable than the free enzyme in octane with a half life of 200 days at 45 degrees C, while that of free enzyme is 5.4 days. CLECs in octane is more stable than in acetonitrile.

引用

页码：467 / 469

页数：3

共 11 条

[1] ENZYME CRYSTAL-STRUCTURE IN A NEAT ORGANIC-SOLVENT [J].

FITZPATRICK, PA ;

STEINMETZ, ACU ;

RINGE, D ;

KLIBANOV, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (18) :8653-8657

[2] ENZYME MEMORY - WHAT IS REMEMBERED AND WHY [J].

KLIBANOV, AM .

NATURE, 1995, 374 (6523) :596-596

[3]

Koskinen AMP., 1996, Enzymatic reactions in organic media, DOI DOI 10.1007/978-94-011-0611-5_11

[4]

NIEDHART DJ, 1988, PROTEIN ENG, V2, P271

[5]

Noritomi H, 1996, BIOTECHNOL BIOENG, V51, P95, DOI 10.1002/(SICI)1097-0290(19960705)51:1<95::AID-BIT11>3.0.CO

[6]

2-3

[7] INTERMOLECULAR CROSS LINKING OF PROTEIN IN CRYSTALLINE STATE - CARBOXYPEPTIDASE-A [J].

QUIOCHO, FA ;

RICHARDS, FM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 52 (03) :833-+

[8] The mechanistic dissection of the plunge in enzymatic activity upon transition from water to anhydrous solvents [J].

Schmitke, JL ;

Wescott, CR ;

Klibanov, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (14) :3360-3365

[9] ENZYME THERMOINACTIVATION IN ANHYDROUS ORGANIC-SOLVENTS [J].

VOLKIN, DB ;

STAUBLI, A ;

LANGER, R ;

KLIBANOV, AM .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 37 (09) :843-853

[10] Rational control of enzymatic enantioselectivity through solvation thermodynamics [J].

Wescott, CR ;

Noritomi, H ;

Klibanov, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (43) :10365-10370

← 1 2 →