The Multi-PDZ domain protein MUPP1 as a lipid raft-associated scaffolding protein controlling the acrosome reaction in mammalian spermatozoa

被引:30
作者
Ackermann, Frauke [1 ]
Zitranski, Nele [1 ]
Heydecke, Diana [1 ]
Wilhelm, Beate [2 ]
Gudermann, Thomas [1 ]
Boekhoff, Ingrid [1 ]
机构
[1] Univ Marburg, Dept Pharmacol & Toxicol, D-35032 Marburg, Germany
[2] Univ Marburg, Dept Anat & Cell Biol, D-35032 Marburg, Germany
关键词
D O I
10.1002/jcp.21272
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The success of acrosomal exocytosis, a complex process with a variety of interrelated steps, relies on the coordinated interaction of participating signaling molecules. Since scaffolding proteins are known to spatially organize sequential signaling pathways, we examined whether the Multi-PDZ domain protein MUPP1, recently identified in mammalian spermatozoa, is functionally active in controlling acrosomal secretion in mammalian sperm cells. To address this question, permeabilized mouse sperm were loaded with inhibitory antibodies against MUPP1 as well as with a photosensitive Ca2+ chelator which allows a controlled release of acrosomal Ca2+. The results revealed that MUPP1 controls initial tethering and docking of the acrosomal vesicle, whereas syntaxin 2, at-SNARE protein also expressed in the acrosomal cap of mammalian spermatozoa, appears to take part in the final process of acrosomal fusion. Interestingly, using immunogold electron microscopy, it was found that MUPP1 is detectable in the region of the periacrosomal membrane. Furthermore, in isolated detergent-insoluble glycolipid-enriched membrane domains from epididymal spermatozoa, MUPP1 was found to show a striking association with the Triton X-100 insoluble membrane fraction, which did not change significantly upon sperm capacitation or partial chemical extraction of cholesterol. This evidence points to a role of MUPP1 as a membrane raft-associated molecular organizer, and suggests that mammalian spermatozoa may use a scaffolding protein and distinct membrane subdomains to spatially organize components involved in the process of acrosomal exocytosis.
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收藏
页码:757 / 768
页数:12
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