Cytokine gene activity in AML cells in vivo in patients

被引：18

作者：

Gao, XZ ^{[1
]}

Bi, SC ^{[1
]}

Copra, H ^{[1
]}

Devemy, E ^{[1
]}

Venugopal, P ^{[1
]}

Li, BR ^{[1
]}

Hsu, WT ^{[1
]}

Loew, J ^{[1
]}

Galvez, A ^{[1
]}

Gregory, S ^{[1
]}

Yang, J ^{[1
]}

Horvath, E ^{[1
]}

Preisler, HD ^{[1
]}

机构：

[1] Rush Presbyterian St Lukes Med Ctr, Rush Canc Inst, Chicago, IL 60612 USA

来源：

LEUKEMIA RESEARCH | 1998年 / 22卷 / 05期

关键词：

AML; cytokines; IL-1; beta; TNF; IL-1ra; GM-CSF; IL-6;

D O I：

10.1016/S0145-2126(98)00024-1

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The proliferation of acute myelogenous leukemia cells is dependent upon cytokine stimulation. Additionally, there is a body of literature which reports that leukemia cells produce GMCSF, IL6, and other cytokines. The study reported here, using an rt-multiplex polymerase method, determined the presence or absence of transcripts in freshly obtained AML cells for the following cytokine or cytokine-related genes: IL 1 beta, IL1ra, TNF alpha, GMCSF, IL6, fit 3, and hSCF. This demonstrated that leukemia cell populations usually contain transcripts for IL1 beta, TNF alpha, fit 3 and fit 3 ligand in vivo and that transcripts for the other cytokines only appear after the leukemia cells are processed in vitro. The presence of TNFa transcripts appears to be associated with resistance to remission induction therapy. Furthermore, the transcript profile of the leukemia cells can change during remission induction therapy. The data also demonstrate the assessment of cytokine production by leukemia cells after in vitro manipulation should not be extrapolated to the in vivo situation. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：429 / 438

页数：10

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