Peptide profiling in epithelial tumor plasma by the emerging proteomic techniques

被引：24

作者：

Caputo, E

Lombardi, ML

Luongo, V

Moharram, R

Tornatore, P

Pirozzi, G

Guardiola, J

Martin, BA

机构：

[1] CNR, A Buzzati Traverso, IGB, I-80125 Naples, Italy

[2] NIMH, Unit Mol Struct, Bethesda, MD 20892 USA

[3] Immunol Ist Nazl Tumori Fdn G Pascale, I-80131 Naples, Italy

[4] Ciphergen Biosyst, Fremont, CA USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 819卷 / 01期

关键词：

melanoma; breast cancer; plasma peptide component; SELDI-TOF MS;

D O I：

10.1016/j.jchromb.2005.01.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The plasma peptide component (PPC) from ten melanoma (Mel), breast cancer (BC) and healthy individuals was examined by a combination of RP-HPLC, surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOFMS) and tandem mass spectrometry. A three peak pattern (2023, 2039, 2053.5 m/z) was primarily observed in melanoma. Two peaks (2236.1 and of 2356.3 m/z) were found only in BC samples. Fibrinogen alpha and inter-alpha-trypsin inhibitor heavy chain H4 fragments were absent in both tumor samples. (c) 2005 Elsevier B.V. All rights reserved.

引用

收藏

页码：59 / 66

页数：8

相关论文

共 24 条

[1] The human plasma proteome - History, character, and diagnostic prospects [J].

Anderson, NL ;

Anderson, NG .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (11) :845-867

[2] Matrix assisted laser desorption/ionization coupled with quadrupole/orthogonal acceleration time-of-flight mass spectrometry for protein discovery, identification, and structural analysis [J].

Baldwin, MA ;

Medzihradszky, KF ;

Lock, CM ;

Fisher, B ;

Settineri, TA ;

Burlingame, AL .

ANALYTICAL CHEMISTRY, 2001, 73 (08) :1707-1720

[3] Methods for on-chip protein analysis [J].

Caputo, E ;

Moharram, R ;

Martin, BM .

ANALYTICAL BIOCHEMISTRY, 2003, 321 (01) :116-124

[4] PURIFICATION AND CHARACTERIZATION OF A NOVEL GLYCOPROTEIN WHICH HAS SIGNIFICANT HOMOLOGY TO HEAVY-CHAINS OF INTER-ALPHA-TRYPSIN INHIBITOR FAMILY FROM HUMAN PLASMA [J].

CHOIMIURA, NH ;

SANO, Y ;

ODA, E ;

NAKANO, Y ;

TOBE, T ;

YANAGISHITA, T ;

TANIYAMA, M ;

KATAGIRI, T ;

TOMITA, M .

JOURNAL OF BIOCHEMISTRY, 1995, 117 (02) :400-407

[5] RAPID MASS-SPECTROMETRIC PEPTIDE SEQUENCING AND MASS MATCHING FOR CHARACTERIZATION OF HUMAN-MELANOMA PROTEINS ISOLATED BY 2-DIMENSIONAL PAGE [J].

CLAUSER, KR ;

HALL, SC ;

SMITH, DM ;

WEBB, JW ;

ANDREWS, LE ;

TRAN, HM ;

EPSTEIN, LB ;

BURLINGAME, AL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (11) :5072-5076

[6]

Doolittle R F, 1973, Adv Protein Chem, V27, P1, DOI 10.1016/S0065-3233(08)60446-5

[7]

FELDINGHABERMANN B, 1992, J BIOL CHEM, V267, P5070

[8]

Ferrari L, 2000, RAPID COMMUN MASS SP, V14, P1149, DOI 10.1002/1097-0231(20000715)14:13<1149::AID-RCM2>3.0.CO

[9]

2-Y

[10] IMMUNOVISUALIZATION OF FIBRINOGEN-A-ALPHA-CHAIN HETEROGENEITY IN NORMAL PLASMA AND PLASMA FROM PATIENTS WITH DIC OR ON STREPTOKINASE THERAPY [J].

GRON, B ;

BENNICK, A ;

NIEUWENHUIZEN, W ;

BJORNSEN, S ;

BROSSTAD, F .

THROMBOSIS RESEARCH, 1988, 52 (05) :413-424