Distribution of protein phosphatases type 1 and 2A in RINm5F cells

被引：4

作者：

Cartus, T ^{[1
]}

Heurich, RO ^{[1
]}

Drews, G ^{[1
]}

Ammon, HPT ^{[1
]}

机构：

[1] Univ Tubingen, Inst Pharmaceut Sci, Dept Pharmacol, D-72076 Tubingen, Germany

来源：

REGULATORY PEPTIDES | 1998年 / 77卷 / 1-3期

关键词：

serine/threonine protein phosphatases; RINm5F cells; okadaic acid;

D O I：

10.1016/S0167-0115(98)00043-3

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In the insulin producing cell line RINm5F distribution of serine/threonine specific protein phosphatases type 1 (PPI) and 2A (PP2A) was studied. Using different agents which inhibit or stimulate PPI and PP2A we found that in membrane and nuclear fractions phosphatase activity was inhibited by okadaic acid (OA), protamine, heparin, and inhibitor-2 in a concentration-dependent manner. C-2-ceramide had no effect. In the cytosolic fraction the inhibitory effect of okadaic acid was tenfold higher. Protamine stimulated phosphatase activity at low concentrations and became inhibitory at higher concentrations. Inhibitor-2 and heparin caused a decrease in phosphatase activity whereas C-2-ceramide led to a slight activation. The data suggest that in membrane and nuclear fractions of RINm5F cells predominantly PPI is present, whereas in the cytosol PPI as well as PP2A can be detected. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：77 / 81

页数：5

共 34 条

[1] INHIBITOR-2 FUNCTIONS LIKE A CHAPERONE TO FOLD 3 EXPRESSED ISOFORMS OF MAMMALIAN PROTEIN PHOSPHATASE-1 INTO A CONFORMATION WITH THE SPECIFICITY AND REGULATORY PROPERTIES OF THE NATIVE ENZYME [J].

ALESSI, DR ;

STREET, AJ ;

COHEN, P ;

COHEN, PTW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 213 (03) :1055-1066

[2] ACTIVATION OF PROTEIN-KINASES AND INHIBITION OF PROTEIN PHOSPHATASES PLAY A CENTRAL ROLE IN THE REGULATION OF EXOCYTOSIS IN MOUSE PANCREATIC BETA-CELLS [J].

AMMALA, C ;

ELIASSON, L ;

BOKVIST, K ;

BERGGREN, PO ;

HONKANEN, RE ;

SJOHOLM, A ;

RORSMAN, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (10) :4343-4347

[3]

Ammon HPT, 1996, N-S ARCH PHARMACOL, V354, P95

[4]

ARKHAMMAR P, 1994, J BIOL CHEM, V269, P2743

[5] STIMULUS-SECRETION COUPLING IN PANCREATIC BETA-CELLS [J].

ASHCROFT, FM ;

PROKS, P ;

SMITH, PA ;

AMMALA, C ;

BOKVIST, K ;

RORSMAN, P .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 55 :54-65

[6] PROTEIN-PHOSPHORYLATION IN THE REGULATION OF INSULIN-SECRETION AND BIOSYNTHESIS [J].

ASHCROFT, SJH ;

HUGHES, SJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1990, 18 (01) :116-118

[7] INHIBITORY EFFECT OF A MARINE-SPONGE TOXIN, OKADAIC ACID, ON PROTEIN PHOSPHATASES - SPECIFICITY AND KINETICS [J].

BIALOJAN, C ;

TAKAI, A .

BIOCHEMICAL JOURNAL, 1988, 256 (01) :283-290

[8] THE STRUCTURE, ROLE, AND REGULATION OF TYPE-1 PROTEIN PHOSPHATASES [J].

BOLLEN, M ;

STALMANS, W .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1992, 27 (03) :227-281

[9]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[10] THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES [J].

COHEN, P .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :453-508

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