Cloning and characterization of RAD17, a gene controlling cell cycle responses to DNA damage in Saccharomyces cerevisiae

被引:44
作者
Siede, W
Nusspaumer, G
Portillo, V
Rodriguez, R
Friedberg, EC
机构
[1] UNIV TEXAS,SW MED CTR,DEPT PATHOL,LAB MOL PATHOL,DALLAS,TX 75235
[2] IIBCE,DIV MOLEC BIOL,MONTEVIDEO,URUGUAY
[3] UNIV REPUBLICA,FAC CIENCIAS,DEPT BIOCHIM,MONTEVIDEO,URUGUAY
关键词
D O I
10.1093/nar/24.9.1669
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mutants of the yeast Saccharomyces cerevisiae defective in the RAD17 gene are sensitive to ultraviolet (UV) and gamma radiation and manifest a defect in G(2) arrest following radiation treatment. We have cloned the RAD17 gene by complementation of the UV sensitivity of a rad17-1 mutant and identified an ORF of 1.2 kb encoding a predicted gene product of 45.4 kDa with homology to the Schizosaccharomyces pombe rad1(+) gene product and to Ustilago maydis Red, a known 3'-->5' exonuclease. The RAD17 transcript is cell cycle regulated, with maximum steady-state levels during late G(1). The rad17-1 mutation represents a missense mutation that maps to a conserved region of the gene. A rad17 disruption mutant grows normally and manifests levels of UV sensitivity similar that of the rad17-1 strain. As previously observed with other genes involved in G(2) arrest (such as RADS and RAD24), RAD17 regulates radiation-induced G(1) checkpoints at at least two possible arrest stages. One is equivalent to or upstream of START, the other at or downstream of the Cdc4 execution point. However, the temperature sensitivity of the cell cycle mutant dna1-1 (a G(1) arrest mutant) is not influenced by inactivation of RAD17.
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页码:1669 / 1675
页数:7
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