Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates

被引：49

作者：

Morais, Luis

Carvalho, Maria Da Gloria

Roca, Anna

Flannery, Brendan

Mandomando, Inacio

Soriano-Gabarro, Montserrat

Sigauque, Betuel

Alonso, Pedro

Beall, Bernard ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Div Bacterial Dis, Resp Dis Branch, Atlanta, GA 30333 USA

[2] Minist Saude, Ctr Invest Saude Manhica CISM, Maputo, Mozambique

[3] Univ Barcelona, Hosp Clin IDIBAPS, Ctr Salut Intl CSI, Barcelona, Spain

[4] Inst Nacl Saude Minist Saude, Maputo, Mozambique

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2007年 / 56卷 / 09期

关键词：

D O I：

10.1099/jmm.0.47346-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8 of 153 isolates; 126 (82.4 %) were identified within two reactions. This approach in developing countries would require minimal training and could provide useful serotype information without requiring transport of specimens.

引用

收藏

页码：1181 / 1184

页数：4

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