Coordinate regulation of lymphocyte-endothelial interactions by pregnancy-associated hormones

被引:51
作者
Chantakru, S
Wang, WC
van den Heuvel, M
Bashar, HS
Simpson, A
Chen, Q
Croy, BA
Evans, SS
机构
[1] Roswell Pk Canc Inst, Dept Immunol, Buffalo, NY 14263 USA
[2] Univ Guelph, Dept Biomed Sci, Guelph, ON N1G 2W1, Canada
基金
加拿大健康研究院;
关键词
D O I
10.4049/jimmunol.171.8.4011
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Precursors of uterine NK cells home to the uterus during early pregnancy from multiple lymphohemopoietic sources. In mouse uterine tissue, pregnancy markedly up-regulates both L-selectin- and alpha(4) integrin-dependent adhesion pathways for circulating human CD56(bright) cells, the phenotype of human uterine NK cells. Based on roles for these adhesion molecules in. lymphocyte homing, we examined effects of pregnancy or the steroid hormones 17beta-estradiol or progesterone on lymphocyte-endothelial interactions in secondary lymphoid tissues and in uterus. From preimplantation gestation day 3, specialized high endothelial venules in peripheral lymph nodes and Peyer's patches supported elevated L-selectin and alpha(4) integrin-dependent lymphocyte adhesion under shear, throughout pregnancy, as compared with high endothelial venules of virgin or postpartum donors. Squamous endothelium from nonlymphoid tissue was not affected. Pregnancy-equivalent endothelial responses were observed in lymph nodes and Peyer's patches from ovariectomized mice receiving 17beta-estradiol and/or progesterone replacement therapy. Adhesion of human CD56(bright) cells to uteri from pregnant or hormone-treated ovariectomized mice was enhanced through L-selectin- and a, integrin-dependent mechanisms and involved multiple vascular adhesion molecules including mucosal addressin cell adhesion molecule-1, VCAM-1, and peripheral lymph node addressin. Analysis of Tie2-green fluorescence protein transgenic mice demonstrated that CD56(bright) Cells adhered primarily to vascular endothelium within the decidua basalis. Microdomain localization of adhesion involving large clusters of lymphocytes was induced on uteri from natural matings, but not pseudopregnancy. Steroid hormones also bad independent effects on L-selectin function in splenic lymphocytes that mimicked physiological stimulation induced by pregnancy or fever-range temperatures. These results provide the first evidence for coordinated, organ-specific, steroid hormone-induced changes in lymphocyte homing mechanisms that could contribute to local and systemic immune responses during pregnancy.
引用
收藏
页码:4011 / 4019
页数:9
相关论文
共 66 条
[1]  
ANDREW DP, 1994, J IMMUNOL, V153, P3847
[2]  
ANSELL JD, 1978, CLIN EXP IMMUNOL, V31, P397
[3]   Interferon γ contributes to initiation of uterine vascular modification, decidual integrity, and uterine natural killer cell maturation during normal murine pregnancy [J].
Ashkar, AA ;
Di Santo, JP ;
Croy, BA .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 192 (02) :259-269
[4]   Modulation of endothelial cell expression of ICAM-1, E-selectin, and VCAM-1 by beta-estradiol, progesterone, and dexamethasone [J].
Aziz, KE ;
Wakefield, D .
CELLULAR IMMUNOLOGY, 1996, 167 (01) :79-85
[5]   Expression of matrix metalloproteinases 2 and 9 in the mouse uterus during implantation and oil-induced decidualization [J].
Bany, BM ;
Harvey, MB ;
Schultz, GA .
JOURNAL OF REPRODUCTION AND FERTILITY, 2000, 120 (01) :125-134
[6]   ALPHA-4-BETA-7-INTEGRIN MEDIATES LYMPHOCYTE BINDING TO THE MUCOSAL VASCULAR ADDRESSIN MADCAM-1 [J].
BERLIN, C ;
BERG, EL ;
BRISKIN, MJ ;
ANDREW, DP ;
KILSHAW, PJ ;
HOLZMANN, B ;
WEISSMAN, IL ;
HAMANN, A ;
BUTCHER, EC .
CELL, 1993, 74 (01) :185-195
[7]   Functional analysis of murine uterine natural killer cells genetically devoid of oestrogen receptors [J].
Borzychowski, AM ;
Chantakru, S ;
Minhas, K ;
Paffaro, VA ;
Yamada, AT ;
He, H ;
Korach, KS ;
Croy, BA .
PLACENTA, 2003, 24 (04) :403-411
[8]   Quantitative analysis of adhesion molecules on cellular constituents of the human uterine microenvironment under the influence of estrogen and progesterone [J].
Brackin, MN ;
Cruse, JM ;
Lewis, RE ;
Hines, RS ;
Stopple, JA ;
Cowan, BD .
EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2002, 72 (02) :91-114
[9]   THE ROLE OF INTEGRINS IN ADHESION OF DECIDUAL NK CELLS TO EXTRACELLULAR-MATRIX AND DECIDUAL STROMAL CELLS [J].
BURROWS, TD ;
KING, A ;
LOKE, YW .
CELLULAR IMMUNOLOGY, 1995, 166 (01) :53-61
[10]   Lymphocyte trafficking and regional immunity [J].
Butcher, EC ;
Williams, M ;
Youngman, K ;
Rott, L ;
Briskin, M .
ADVANCES IN IMMUNOLOGY, VOL. 72, 1999, 72 :209-253