Identification of humoral antigenic determinants in the hepatitis C virus NS3 protein

被引：12

作者：

Hwang, LH

Yang, PM

Lai, MY

Chiang, BL

Kao, JH

Wang, JT

Lee, SY

Chian, HM

Chi, WK

Chu, YD

Chen, PJ

Chen, DS

机构：

[1] NATL TAIWAN UNIV HOSP,HEPATITIS RES CTR,TAIPEI 100,TAIWAN

[2] NATL TAIWAN UNIV HOSP,DEPT INTERNAL MED,TAIPEI 100,TAIWAN

[3] NATL TAIWAN UNIV,COLL MED,GRAD INST CLIN MED,TAIPEI 10764,TAIWAN

[4] NATL TAIWAN UNIV,GRAD INST MICROBIOL,TAIPEI 10764,TAIWAN

[5] DEV CTR BIOTECHNOL,PROC DEV DIV,TAIPEI,TAIWAN

来源：

JOURNAL OF INFECTIOUS DISEASES | 1996年 / 174卷 / 01期

关键词：

D O I：

10.1093/infdis/174.1.173

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around aa 1250/1251), A (within aa 1250-1334), A/B (around aa 1323 and 1334), and B/C (around aa 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis (10% vs. 46%, chi(2) = 12.05, P <.01). The results suggest that region A might be a potential prognostic marker to differentiate chronic hepatitis from cirrhosis.

引用

收藏

页码：173 / 176

页数：4

相关论文

共 14 条

[1] THE TAIWANESE HEPATITIS-C VIRUS GENOME - SEQUENCE DETERMINATION AND MAPPING THE 5' TERMINI OF VIRAL GENOMIC AND ANTIGENOMIC RNA [J].

CHEN, PJ ;

LIN, MH ;

TAI, KF ;

LIU, PC ;

LIN, CJ ;

CHEN, DS .

VIROLOGY, 1992, 188 (01) :102-113

[2] TRANSIENT IMMUNOGLOBULIN-M ANTIBODY-RESPONSE TO HEPATITIS-C VIRUS CAPSID ANTIGEN IN POSTTRANSFUSION HEPATITIS-C - PUTATIVE SEROLOGICAL MARKER FOR ACUTE VIRAL-INFECTION [J].

CHEN, PJ ;

WANG, JT ;

HWANG, LH ;

YANG, YH ;

HSIEH, CL ;

KAO, JH ;

SHEU, JC ;

LAI, MY ;

WANG, TH ;

CHEN, DS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (13) :5971-5975

[3] DIAGNOSIS OF HEPATITIS-C VIRUS (HCV) INFECTION USING AN IMMUNODOMINANT CHIMERIC POLYPROTEIN TO CAPTURE CIRCULATING ANTIBODIES - REEVALUATION OF THE ROLE OF HCV IN LIVER-DISEASE [J].

CHIEN, DY ;

CHOO, QL ;

TABRIZI, A ;

KUO, C ;

MCFARLAND, J ;

BERGER, K ;

LEE, C ;

SHUSTER, JR ;

NGUYEN, T ;

MOYER, DL ;

TONG, M ;

FURUTA, S ;

OMATA, M ;

TEGTMEIER, G ;

ALTER, H ;

SCHIFF, E ;

JEFFERS, L ;

HOUGHTON, M ;

KUO, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10011-10015

[4] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[5] LOCALIZATION AND REACTIVITY OF AN IMMUNODOMINANT DOMAIN IN THE NS3 REGION OF HEPATITIS-C VIRUS [J].

CLAEYS, H ;

VOLCKAERTS, A ;

MERTENS, W ;

LIANG, Z ;

FITEN, P ;

OPDENAKKER, G .

JOURNAL OF MEDICAL VIROLOGY, 1995, 45 (03) :273-281

[6] CHARACTERIZATION OF THE HEPATITIS-C VIRUS-ENCODED SERINE PROTEINASE - DETERMINATION OF PROTEINASE-DEPENDENT POLYPROTEIN CLEAVAGE SITES [J].

GRAKOUI, A ;

MCCOURT, DW ;

WYCHOWSKI, C ;

FEINSTONE, SM ;

RICE, CM .

JOURNAL OF VIROLOGY, 1993, 67 (05) :2832-2843

[7] MOLECULAR-BIOLOGY OF THE HEPATITIS-C VIRUSES - IMPLICATIONS FOR DIAGNOSIS, DEVELOPMENT AND CONTROL OF VIRAL DISEASE [J].

HOUGHTON, M ;

WEINER, A ;

HAN, J ;

KUO, G ;

CHOO, QL .

HEPATOLOGY, 1991, 14 (02) :381-388

[8] AN ASSAY FOR CIRCULATING ANTIBODIES TO A MAJOR ETIOLOGIC VIRUS OF HUMAN NON-A, NON-B-HEPATITIS [J].

KUO, G ;

CHOO, QL ;

ALTER, HJ ;

GITNICK, GL ;

REDEKER, AG ;

PURCELL, RH ;

MIYAMURA, T ;

DIENSTAG, JL ;

ALTER, MJ ;

STEVENS, CE ;

TEGTMEIER, GE ;

BONINO, F ;

COLOMBO, M ;

LEE, WS ;

KUO, C ;

BERGER, K ;

SHUSTER, JR ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :362-364

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10] SIGNIFICANCE OF THE IMMUNE-RESPONSE TO A MAJOR, CONFORMATIONAL B-CELL EPITOPE OS THE HEPATITIS-C VIRUS NS3 REGION DEFINED BY A HUMAN MONOCLONAL-ANTIBODY [J].

MONDELL, MU ;

CERINO, A ;

BOENDER, P ;

OUDSHOORN, P ;

MIDDELDORP, J ;

FIPALDINI, C ;

LAMONICA, N ;

HABETS, W .

JOURNAL OF VIROLOGY, 1994, 68 (08) :4829-4836