Effect of the three-dimensional organization of liver cells on the biogenesis of the γ-glutamyltransferase fraction pattern

被引：2

作者：

Corti, Alessandro ^{[1
]}

Fierabracci, Vanna ^{[1
]}

Caponi, Laura ^{[1
]}

Paolicchi, Aldo ^{[1
]}

Lorenzini, Evelina ^{[1
]}

Campani, Daniela ^{[2
]}

Belcastro, Eugenia ^{[1
]}

Franzini, Maria ^{[1
,3
]}

机构：

[1] Univ Pisa, Dept Translat Res & New Technol Med & Surg, Pisa, Italy

[2] Univ Pisa, Dept Surg Med Mol Pathol & Emergency Med, Pisa, Italy

[3] Fdn G Monasterio, CNR Reg Toscana, Pisa, Italy

来源：

BIOMARKERS | 2016年 / 21卷 / 05期

关键词：

Bile acids; gel-filtration chromatography; HepG2; cells; liver parenchyma; spheroids; COLON-CARCINOMA CELLS; NORMAL HUMAN TISSUE; CARDIOVASCULAR-DISEASE; METABOLIC SYNDROME; HEPG2; SPHEROIDS; B-GGT; TRANSPEPTIDASE; RISK; HEART; SERUM;

D O I：

10.3109/1354750X.2016.1153719

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Context Four gamma-glutamyltransferase (GGT) fractions with different molecular weights (big-, medium-, small-and free-GGT) are detectable in human plasma. Objective Verify if liver cells can release all four GGT fractions and if the spatial cell organization influences their release. Methods Hepatoma (HepG2) and melanoma (Me665/2/60) cells were cultured as monolayers or spheroids. GGT released in culture media was analysed by gel-filtration chromatography. Results HepG2 and Me665/2/60 monolayers released the b-GGT fraction, while significative levels of s-GGT and f-GGT were detectable only in media of HepG2-spheroids. Bile acids alone or in combination with papain promoted the conversion of b-GGT in s-GGT or f-GGT, respectively. Conclusions GGT is usually released as b-GGT, while s-GGT and f-GGT are likely to be produced in the liver extracellular environment by the combined action of bile acids and proteases.

引用

页码：441 / 448

页数：8

共 42 条

[1]

Hepascore: An accurate validated predictor of liver fibrosis in chronic hepatitis C infection [J].