Protective effect of mannitol, glucose-fructose-sucrose-maltose mixture, and natural honey hyperosmolar solutions against ethanol-induced gastric mucosal damage in rats

被引:22
作者
Gharzouli, K [1 ]
Gharzouli, A [1 ]
Amira, S [1 ]
Khennouf, S [1 ]
机构
[1] Univ Ferhat Abbas, Fac Sci, Dept Biol, Setif, Algeria
关键词
acid secretion; cytoprotection; ethanol; experimental gastric ulcer; gastric emptying; honey; mannitol; osmolality;
D O I
10.1078/0940-2993-00175
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Background: We have previously shown that natural honey is able to protect the rat stomach against acute ethanol- and indomethacin-induced lesions. The present investigations were undertaken to examine the role of intraluminal osmolality in this protective effect. Methods: Mannitol, glucose-fructose-sucrose-maltose mixture (GFSM) and natural honey (300, 600, 1800 mOsmol/kg water) were given orally to rats 30 min before administration of 70% ethanol for a further 15-min period. Lesions area of the excised stomachs were evaluated. Pylorus-ligated stomachs were filled with mannitol, GFSM mixture and honey (1800 mOsmol/kg water) to test the effect of the hyperosmolar solutions on gastric fluid content and acid secretion. The rate of gastric emptying of the three rest solutions (1800 mOsmol/kg) was measured by the phenol red method. Results: Intragastric administration of mannitol. GFSM mixture or honey prevented the formation of mucosal lesions in an osmolality-dependent manner. Using the pylorus-ligated stomach model, the test solutions led to a net increase of luminal fluid volume without affecting acid content. Hyperosmolar solutions presented a delayed gastric emptying if compared to a nonnutrient solution made of carboxymethyl cellulose. Conclusions: The observed results suggest that hyperosmolar solutions can prevent the formation of hemorrhagic lesions by luminal dilution of the necrotising agent and acid, an effect which may be potentiated by a lowered gastric emptying rate.
引用
收藏
页码:175 / 180
页数:6
相关论文
共 29 条
[1]  
ALI ATM, 1995, SAUDI MED J, V16, P161
[2]  
Ali ATMM, 1996, SAUDI MED J, V17, P301
[3]   PREVENTION OF ETHANOL-INDUCED GASTRIC-LESIONS IN RATS BY NATURAL HONEY, AND ITS POSSIBLE MECHANISM OF ACTION [J].
ALI, ATMM .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1991, 26 (03) :281-288
[4]  
ALI ATMM, 1990, SAUDI MED J, V11, P275
[5]  
ALLEGRETTI M, 1987, IND ALIMENT-ITALY, V26, P566
[6]  
ALSOMAL N, 1994, J ROY SOC MED, V57, P9
[7]   ABDOMINAL SURGERY-INDUCED DELAYED GASTRIC-EMPTYING IN RATS - ROLE OF CRF AND SENSORY NEURONS [J].
BARQUIST, E ;
ZINNER, M ;
RIVIER, J ;
TACHE, Y .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (04) :G616-G620
[8]   GASTRIC-MUCOSA BARRIER [J].
DAVENPORT, HW .
DIGESTION, 1972, 5 (3-4) :162-+
[9]   CLINICAL OBSERVATIONS ON THE WOUND-HEALING PROPERTIES OF HONEY [J].
EFEM, SEE .
BRITISH JOURNAL OF SURGERY, 1988, 75 (07) :679-681
[10]   THE ANTIMICROBIAL SPECTRUM OF HONEY AND ITS CLINICAL-SIGNIFICANCE [J].
EFEM, SEE ;
UDOH, KT ;
IWARA, CI .
INFECTION, 1992, 20 (04) :227-229