Exopolysaccharide biosynthesis in Lactococcus lactis NIZO B40:: Functional analysis of the glycosyltransferase genes involved in synthesis of the polysaccharide backbone

被引：71

作者：

van Kranenburg, R ^{[1
]}

van Swam, II ^{[1
]}

Marugg, JD ^{[1
]}

Kleerebezem, M ^{[1
]}

de Vos, WM ^{[1
]}

机构：

[1] NIZO Food Res, Microbial Ingredients Sect, NL-6710 BA Ede, Netherlands

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 01期

关键词：

D O I：

10.1128/JB.181.1.338-340.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We used homologous and heterologous expression of the glycosyltransferase genes of the Lactococcus lactis NIZO B40 eps gene cluster to determine the activity and substrate specificities of the encoded enzymes and established the order of assembly of the trisaccharide backbone of the exopolysaccharide repeating unit. EpsD links glucose-1-phosphate from UDP-glucose to a lipid carrier, EpsE and EpsF link glucose from UDP-glucose to lipid-linked glucose, and EpsG links galactose from UDP-galactose to lipid-linked cellobiose. Furthermore, EpsJ appeared to be involved in EPS biosynthesis as a galactosyl phosphotransferase or an enzyme which releases the backbone oligosaccharide from the lipid carrier.

引用

页码：338 / 340

页数：3

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