Rapid, Label-Free, Electrical Whole Blood Bioassay Based on Nanobiosensor Systems

被引:71
作者
Chang, Hsiao-Kang [1 ]
Ishikawa, Fumiaki N. [1 ]
Zhang, Rui [2 ]
Datar, Ram [3 ]
Cote, Richard J. [3 ]
Thompson, Mark E. [2 ]
Zhou, Chongwu [1 ]
机构
[1] Univ So Calif, Dept Elect Engn, Los Angeles, CA 90089 USA
[2] Univ So Calif, Dept Chem, Los Angeles, CA 90089 USA
[3] Univ Miami, Dept Pathol, Miami, FL 33136 USA
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
biosensor; nanowire; whole blood detection; cancer biomarker detection; NANOWIRE TRANSISTORS; SERUM BIOMARKER; ELECTRONIC-PROPERTIES; DIAGNOSTIC MARKERS; OVARIAN CANCERS; IN2O3; NANOWIRES; PANEL; CHEMOTHERAPY; PERFORMANCE; PREDICTION;
D O I
10.1021/nn2035796
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Biomarker detection based on nanowire biosensors has attracted a significant amount of research effort in recent years. However, only very limited research work has been directed toward biomarker detection directly from physiological fluids mainly because of challenges caused by the complexity of media. This limitation significantly reduces the practical impact generated by the aforementioned nanobiosensors. In this study, we demonstrate an In2O3 nanowire-based biosensing system that is capable of performing rapid, label-free, electrical detection of cancer biomarkers directly from human whole blood collected by a finger prick. Passivating the nanowire surface successfully blocked the signal induced by nonspecific binding when performing active measurement in whole blood. Passivated devices showed markedly smaller signals induced by nonspecific binding of proteins and other biomaterials in serum and higher sensitivity to target biomarkers than bare devices. The detection limit of passivated sensors for biomarkers in whole blood was similar to the detection limit for the same analyte in purified buffer solutions at the same ionic strength, suggesting minimal decrease In device performance in the complex media. We then demonstrated detection of multiple cancer biomarkers with high reliability at clinically meaningful concentrations from whole blood collected by a finger pick using this sensing system.
引用
收藏
页码:9883 / 9891
页数:9
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