Impact of replicate types on proteomic expression analysis

被引：124

作者：

Karp, NA

Spencer, M

Lindsay, H

O'Dell, K

Lilley, KS

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[2] Dalhousie Univ, Dept Math & Stat, Halifax, NS, Canada

[3] Dalhousie Univ, Dept Biochem & Mol Biol, Halifax, NS, Canada

[4] Univ Glasgow, IBLS Div Mol Genet, Glasgow, Lanark, Scotland

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 05期

关键词：

expression analysis; proteomics; differential gel electrophoresis; replication; nested ANOVA;

D O I：

10.1021/pr050084g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In expression proteomics, the samples utilized within an experimental design may include technical, biological, or pooled replicates. This manuscript discusses various experimental designs and the conclusions that can be drawn from them. Specifically, it addresses the impact of mixing replicate types on the statistical analysis which can be performed. This study focuses on difference gel electrophoresis (DiGE), but the issues are equally applicable to all quantitative methodologies assessing relative changes in protein expression.

引用

页码：1867 / 1871

页数：5

共 27 条

[1] A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard [J].