Functional identification of α1-giardin as an annexin of Giardia lamblia

被引：18

作者：

Bauer, B ^{[1
]}

Engelbrecht, S ^{[1
]}

Bakker-Grunwald, T ^{[1
]}

Scholze, H ^{[1
]}

机构：

[1] Univ Osnabruck, Dept Biol Chem, D-49069 Osnabruck, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 173卷 / 01期

关键词：

Giardia lamblia; giardin; annexin; calcium binding; phospholipid binding;

D O I：

10.1016/S0378-1097(99)00064-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A protein with a relative molecular mass of 31 kDa was specifically extracted by EGTA from a detergent-insoluble fraction of Giardia lamblia. N-terminal sequencing showed this protein to be identical to alpha 1-giardin, a component of the ventral disc which, based on its predicted amino acid sequence, has been classified as annexin XIX. Purified alpha 1-giardin associated with multilamellar phosphatidyl serine-containing vesicles in a Ca2+-dependent manner, confirming that it is a functional annexin. Molecular modelling of the amino acid sequence of the giardial annexin into the X-ray structure of annexin V suggests that the Ca2+-binding sites, which, as in other annexins, are all located on the convex surface of the molecule, are of the low-affinity type III. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：147 / 153

页数：7

共 18 条

[1]

Benz J, 1997, BIOL CHEM, V378, P177

[2] ISOLATION AND CHARACTERIZATION OF 2 NOVEL CALCIUM-DEPENDENT PHOSPHOLIPID-BINDING PROTEINS FROM BOVINE LUNG [J].

BOUSTEAD, CM ;

WALKER, JH ;

GEISOW, MJ .

FEBS LETTERS, 1988, 233 (02) :233-238

[3] CALCIUM-CHANNEL ACTIVITY OF PURIFIED HUMAN SYNEXIN AND STRUCTURE OF THE HUMAN SYNEXIN GENE [J].

BURNS, AL ;

MAGENDZO, K ;

SHIRVAN, A ;

SRIVASTAVA, M ;

ROJAS, E ;

ALIJANI, MR ;

POLLARD, HB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (10) :3798-3802

[4] STRUCTURE-FUNCTION CORRELATIONS OF CALCIUM-BINDING AND CALCIUM-CHANNEL ACTIVITIES BASED ON 3-DIMENSIONAL MODELS OF HUMAN ANNEXIN-I, ANNEXIN-II, ANNEXIN-III, ANNEXIN-V AND ANNEXIN-VII [J].

CHEN, JM ;

SHELDON, A ;

PINCUS, MR .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1993, 10 (06) :1067-&

[5]

CROSSLEY R, 1983, J CELL SCI, V59, P81

[6]

Douglas M, 1979, Methods Enzymol, V56, P58

[7] CA2+-DEPENDENT PHOSPHOLIPID AND ARACHIDONIC-ACID BINDING BY THE PLACENTAL ANNEXIN-VI AND ANNEXIN-IV [J].

EDWARDS, HC ;

CRUMPTON, MJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 198 (01) :121-129

[8] Annexins and membrane dynamics [J].

Gerke, V ;

Moss, SE .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1997, 1357 (02) :129-154

[9] Cell biology of the primitive eukaryote Giardia lamblia [J].

Gillin, FD ;

Reiner, DS ;

McCaffery, JM .

ANNUAL REVIEW OF MICROBIOLOGY, 1996, 50 :679-705

[10] THE CRYSTAL AND MOLECULAR-STRUCTURE OF HUMAN ANNEXIN-V, AN ANTICOAGULANT PROTEIN THAT BINDS TO CALCIUM AND MEMBRANES [J].

HUBER, R ;

ROMISCH, J ;

PAQUES, EP .

EMBO JOURNAL, 1990, 9 (12) :3867-3874

← 1 2 →