The mitochondrial type II peroxiredoxin F is essential for redox homeostasis and root growth of Arabidopsis thaliana under stress

被引:199
作者
Finkemeier, I
Goodman, M
Lamkemeyer, P
Kandlbinder, A
Sweetlove, LJ
Dietz, KJ
机构
[1] Univ Bielefeld, Dept Plant Physiol & Biochem, D-33501 Bielefeld, Germany
[2] Univ Oxford, Dept Plant Sci, Oxford OX1 3RP, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1074/jbc.M413189200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Peroxiredoxins (Prx) have recently moved into the focus of plant and animal research in the context of development, adaptation, and disease, as they function both in antioxidant defense by reducing a broad range of toxic peroxides and in redox signaling relating to the adjustment of cell redox and antioxidant metabolism. At-PrxII F is one of six type II Prx identified in the genome of Arabidopsis thaliana and the only Prx that is targeted to the plant mitochondrion. Therefore, it might be assumed to have functions similar to the human 2-Cys Prx (PRDX3) and type II Prx (PRDX5) and yeast 1-Cys Prx that likewise have mitochondrial localizations. This paper presents a characterization of PrxII F at the level of subcellular distribution, activity, and reductive regeneration by mitochondrial thioredoxin and glutaredoxin. By employing tDNA insertion mutants of A. thaliana lacking expression of AtprxII F (KO-AtPrxII F), it is shown that under optimal environmental conditions the absence of PrxII F is almost fully compensated for, possibly by increases in activity of mitochondrial ascorbate peroxidase and glutathione-dependent peroxidase. However, a stronger inhibition of root growth in KO-AtPrxII F seedlings as compared with wild type is observed under stress conditions induced by CdCl2 as well as after administration of salicylhydroxamic acid, an inhibitor of cyanide-insensitive respiration. Simultaneously, major changes in the abundance of both nuclear and mitochondria-encoded transcripts were observed. These results assign a principal role to PrxII F in antioxidant defense and possibly redox signaling in plants cells.
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收藏
页码:12168 / 12180
页数:13
相关论文
共 79 条
[1]   Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria [J].
Balmer, Y ;
Vensel, WH ;
Tanaka, CK ;
Hurkman, WJ ;
Gelhaye, E ;
Rouhier, N ;
Jacquot, JP ;
Manieri, W ;
Schüurmann, P ;
Droux, M ;
Buchanan, BB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (08) :2642-2647
[2]   Ascorbate biosynthesis in mitochondria is linked to the electron transport chain between complexes III and IV [J].
Bartoli, CG ;
Pastori, GM ;
Foyer, CH .
PLANT PHYSIOLOGY, 2000, 123 (01) :335-343
[3]   Resemblance and dissemblance of Arabidopsis type II peroxiredoxins:: Similar sequences for divergent gene expression, protein localization, and activity [J].
Bréhélin, C ;
Meyer, EH ;
de Souris, JP ;
Bonnard, G ;
Meyer, Y .
PLANT PHYSIOLOGY, 2003, 132 (04) :2045-2057
[4]   Peroxynitrite reductase activity of bacterial peroxiredoxins [J].
Bryk, R ;
Griffin, P ;
Nathan, C .
NATURE, 2000, 407 (6801) :211-215
[5]  
CHAE HZ, 1994, J BIOL CHEM, V269, P27670
[6]   DIMERIZATION OF THIOL-SPECIFIC ANTIOXIDANT AND THE ESSENTIAL ROLE OF CYSTEINE-47 [J].
CHAE, HZ ;
UHM, TB ;
RHEE, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (15) :7022-7026
[7]   Peroxiredoxin III, a mitochondrion-specific peroxidase, regulates apoptotic signaling by mitochondria [J].
Chang, TS ;
Cho, CS ;
Park, S ;
Yu, SQ ;
Kang, SW ;
Rhee, SG .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (40) :41975-41984
[8]   Molecular definition of the ascorbate-glutathione cycle in Arabidopsis mitochondria reveals dual targeting of antioxidant defenses in plants [J].
Chew, O ;
Whelan, J ;
Millar, AH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (47) :46869-46877
[9]   SIMULTANEOUS TARGETING OF PEA GLUTATHIONE-REDUCTASE AND OF A BACTERIAL FUSION PROTEIN TO CHLOROPLASTS AND MITOCHONDRIA IN TRANSGENIC TOBACCO [J].
CREISSEN, G ;
REYNOLDS, H ;
XUE, YB ;
MULLINEAUX, P .
PLANT JOURNAL, 1995, 8 (02) :167-175
[10]   INHIBITION AND STIMULATION OF ROOT RESPIRATION IN PISUM AND PLANTAGO BY HYDROXAMATE - ITS CONSEQUENCES FOR THE ASSESSMENT OF ALTERNATIVE PATH ACTIVITY [J].
DEVISSER, R ;
BLACQUIERE, T .
PLANT PHYSIOLOGY, 1984, 75 (03) :813-817