Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum

被引：32

作者：

Durand, R

Eslahpazire, J

Jafari, S

Delabre, JF

Marmorat-Khuong, A

di Piazza, JP

Le Bras, J

机构：

[1] Hop Bichat Claude Bernard, Parasitol Lab, Assistance Publ Hop Paris, F-75877 Paris 18, France

[2] Univ Paris 05, Ctr Natl Reference Chimiosensibil Paludisme, F-75877 Paris, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 12期

关键词：

D O I：

10.1128/AAC.44.12.3461-3464.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies.

引用

页码：3461 / 3464

页数：4

共 25 条

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